This work focuses on practical approaches to and uses of a revolutionary technology called RNAi for RNA interference. A recently developed research tool, this process allows scientists to knock out or knock down the levels of a gene product, shut off targeted genes in an organism, and then monitor the results within that organism. Leading academic and biotechnology researchers in various fields that utilize this process introduce and explain a specific model system and use of RNA interference as well as necessary background information. Tools for the Arabidopsis, Caenrhabditis, and Drosophila model organism systems are offered, as is an overview of siRNA design and RNA transfection processes in mammalian cells. A discussion of chemical and enzymatic synthesis and preparation of siRNA in vitro is also included.
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Editorial Reviews
Review
A comprehensive guide to RNAi experimentation from the experts in this field! -- Gary Hardiman, Ph.D., Director BIOGEM
About the Author
David Engelke is a professor in the biochemistry department at the University of Michigan.
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4 of 4 people found the following review helpful:
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"Genomics & Proteomics" Book Review 4(4):40, 2004,
By A Customer
This review is from: RNA Interference (RNAi): The Nuts & Bolts of RNAi Technology (Nuts & Bolts Series) (Hardcover)
With the rise and utility of RNA Interference (RNAi) as a useful tool for silencing and manipulating genes, there has been much interest in the specific applications of this technique to various animal models for disease. Because it is a natural phenomenon, RNAi is more robust than other RNA-based methods. However, RNAi pathways do not follow the same rules in different organisms, and therefore, specific information on RNAi methods is essential for each model organism studied.The aim of "RNA Interference (RNAi): Nuts & Bolts of RNAi Technology" by David Engelke and published by DNA Press (www.dnapress.net) is to serve as a guide for practical RNAi use in the laboratory. The book is short on general theory behind RNAi, except to furnish the bare minimum of background information for each chapter. However, the book is rich in specific protocols for the various methods that are described, including suppliers of reagents and advice on modifications and customizations of protocols. Chapters include protocols for RNAi in plants, Drosophila, C. elegans, mammalian systems, mice, and discussions of high-throughput RNAi as well as design, synthesis and preparation of synthetic RNAi. An added bonus each chapter contains an ample list of relevant literature references. Joshua Marcy, www.genpromag.com
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First Sentence:
Cosuppression, post-transcriptional gene silencing (PTGS), and virus-induced gene silencing (VIGS) all describe homology-dependent gene silencing phenomena in plants that involve what is more broadly known as RNA interference (RNAi). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
transfection optimisation, genetic variance detection, silencing potency, knockdown phenotype, internal promoter elements, rev transcripts, gene knockdown, gene expression signatures, specific genetic interference, silencing effect, interferon response, antisense strand, shuttle plasmid, target gene expression, expression cassettes, gene silencing, separation band, transfection reagent, coupling yields, antisense sequences, specific interference, transcription reaction, embryo extract, transfection protocol, cultured mammalian cells Key Phrases - Capitalized Phrases (CAPs): (learn more)
Proc Natl Acad Sci, Proc Notl Acad Sci, Nat Biotechnol, Genes Dev, Nucleic Acids Res, Cold Spring Harbor, Mot Cell, Nat Genet, Curr Biol, Plant Cell, Biol Chem, Cell Sci, Technology Figure, Technology Table, Dev Biol, Molecular Probes, Nat Cell Biol, National Institutes of Health, New England Biolabs, Roche Life Science, Biomol Screen, Molecular Therapy, Proc Natl Acod Sci New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Cosuppression, post-transcriptional gene silencing (PTGS), and virus-induced gene silencing (VIGS) all describe homology-dependent gene silencing phenomena in plants that involve what is more broadly known as RNA interference (RNAi). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
transfection optimisation, genetic variance detection, silencing potency, knockdown phenotype, internal promoter elements, rev transcripts, gene knockdown, gene expression signatures, specific genetic interference, silencing effect, interferon response, antisense strand, shuttle plasmid, target gene expression, expression cassettes, gene silencing, separation band, transfection reagent, coupling yields, antisense sequences, specific interference, transcription reaction, embryo extract, transfection protocol, cultured mammalian cells Key Phrases - Capitalized Phrases (CAPs): (learn more)
Proc Natl Acad Sci, Proc Notl Acad Sci, Nat Biotechnol, Genes Dev, Nucleic Acids Res, Cold Spring Harbor, Mot Cell, Nat Genet, Curr Biol, Plant Cell, Biol Chem, Cell Sci, Technology Figure, Technology Table, Dev Biol, Molecular Probes, Nat Cell Biol, National Institutes of Health, New England Biolabs, Roche Life Science, Biomol Screen, Molecular Therapy, Proc Natl Acod Sci New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
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