RNA Methodologies, Second Edition: A Laboratory Guide for Isolation and Characterization [Paperback]

Robert E. Farrell Jr. (Author)

Book Description

ISBN-10: 0122496957 | ISBN-13: 978-0122496950 | Publication Date: October 8, 1998 | Edition: 2

This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. This edition includes a variety of RNA-based PCR techniques, such as differential display PCR, and transcript quantification by competitive (mimic) PCR. The book focuses on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitate learning and assist in the planning phases of projects. It is a valuable resource for investigators who wish to characterize cellular biochemistry.

Key Features
* Studies cellular biochemistry using RNA as a parameter of gene expression
* Unifies many facets of working with RNA
* Offers multiple isolation strategies directed to the same experimental end
* Explains proper handling, storage, and manipulation of RNA
* Includes classic and contemporary techniques
* Contains fully annotated protocols, as well as rationale for each protocol
* Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Editorial Reviews

Review

"Each of the protocols is written relatively independent of the rest of the text, allowing the reader to immediately begin using a procedure without having to study the text from cover to cover... Very few other molecular biology guide books are available that cover the isolation and manipulation of RNA as exhaustingly as does this one. This book would be highly valuable in any research facility engaged in molecular biology."
--Mark A. Jandreski, Loyola University Medical Center, DOODY'S ELECTRONIC JOURNAL

Book Description

The expanded third edition of this highly successful laboratory manual presents new technologies in RNA interference --This text refers to an out of print or unavailable edition of this title.

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Product Details

• Paperback: 533 pages
• Publisher: Academic Press; 2 edition (October 8, 1998)
• Language: English
• ISBN-10: 0122496957
• ISBN-13: 978-0122496950
• Product Dimensions: 9 x 6.6 x 1 inches
• Shipping Weight: 1.8 pounds
• Average Customer Review: 3.0 out of 5 stars  See all reviews (2 customer reviews)
• Amazon Best Sellers Rank: #4,126,473 in Books (See Top 100 in Books)

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Customer Reviews

Most Helpful Customer Reviews

5 of 5 people found the following review helpful:

4.0 out of 5 stars great methods book, May 15, 2000

By 

"kb-t" - See all my reviews

This review is from: RNA Methodologies, Second Edition: A Laboratory Guide for Isolation and Characterization (Paperback)

This book is exactly what it claims to be--a laboratory guide. It provides methods that are clear, but also explains the reasons for the methods. For example: when is an RNA probe better than a DNA probe and why; when nonisotopic labels can be used effectively. Although there is not a chapter on in situ hybridization, I found the section on probes very helpful. There is also a handy glossary at the end and a primer on RNA at the beginning of the book. Dr Farrell realizes the reader maybe new to the field and so doesn't leave out the basics. However, I believe this book will be helpful to all but the most knowledgable molecular biolgists.

1 of 2 people found the following review helpful:

2.0 out of 5 stars Not advanced and back-dated, December 2, 2004

By 

citu "TM" - See all my reviews

This review is from: RNA Methodologies: A Laboratory Guide for Isolation and Characterization (Plastic Comb)

I am a RNA-chemist and I bought this book from amazon.com to get help for my day-to-day research. The moment I had my first glance at this book I was frustrated. This book has described fairly old methods but no contemporary techniques. This book is not even descrided the techniques in an advanced manner which is expected for a book based on so specific field. I would not recommend this book to anyone who has atleast the basic working knowledge of molecular biology.