RNA Methodologies, Third Edition: A Laboratory Guide for Isolation and Characterization [Plastic Comb]

Robert E. Farrell Jr. (Author)

Book Description

ISBN-10: 0122496965 | ISBN-13: 978-0122496967 | Publication Date: April 15, 2005 | Edition: 3

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.

RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.

* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center
* Includes classic and contemporary techniques
* Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Editorial Reviews

Review

Praise for the third edition
"This third edition of RNA Methodologies is an updated version of a laboratory guide edited for the first time in 1993; it represents an exhaustive collection of tried, tested, and optimized protocols for isolation and chracterization of RNA. The third edition of RNA Methodologies is a well-documented guide for any researcher who has to manage RNA studies. Each chapter of this book provides a rationale to help in the decision-making process...The inclusion of troubleshooting hints and annotations based on personal experience are provided to improve the method(s). This laboratory guide offers protocols on traditional and new technologies, with a focus on how to use the expedient technique for achieving an experimental goal in the study of gene expression."
--Dr. Patrick Pagesy at INSERM for HEMOGLOBIN

Book Description

The expanded third edition of this highly successful laboratory manual presents new technologies in RNA interference

See all Editorial Reviews

Product Details

• Plastic Comb: 688 pages
• Publisher: Academic Press; 3 edition (April 15, 2005)
• Language: English
• ISBN-10: 0122496965
• ISBN-13: 978-0122496967
• Product Dimensions: 8.8 x 6.4 x 1.5 inches
• Shipping Weight: 2.4 pounds (View shipping rates and policies)
• Average Customer Review: 3.0 out of 5 stars  See all reviews (2 customer reviews)
• Amazon Best Sellers Rank: #2,951,408 in Books (See Top 100 in Books)

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Customer Reviews

Most Helpful Customer Reviews

5 of 5 people found the following review helpful:

4.0 out of 5 stars great methods book, May 15, 2000

By 

"kb-t" - See all my reviews

This review is from: RNA Methodologies, Second Edition: A Laboratory Guide for Isolation and Characterization (Paperback)

This book is exactly what it claims to be--a laboratory guide. It provides methods that are clear, but also explains the reasons for the methods. For example: when is an RNA probe better than a DNA probe and why; when nonisotopic labels can be used effectively. Although there is not a chapter on in situ hybridization, I found the section on probes very helpful. There is also a handy glossary at the end and a primer on RNA at the beginning of the book. Dr Farrell realizes the reader maybe new to the field and so doesn't leave out the basics. However, I believe this book will be helpful to all but the most knowledgable molecular biolgists.

1 of 2 people found the following review helpful:

2.0 out of 5 stars Not advanced and back-dated, December 2, 2004

By 

citu "TM" - See all my reviews

This review is from: RNA Methodologies: A Laboratory Guide for Isolation and Characterization (Plastic Comb)

I am a RNA-chemist and I bought this book from amazon.com to get help for my day-to-day research. The moment I had my first glance at this book I was frustrated. This book has described fairly old methods but no contemporary techniques. This book is not even descrided the techniques in an advanced manner which is expected for a book based on so specific field. I would not recommend this book to anyone who has atleast the basic working knowledge of molecular biology.