Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR by presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardization, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion, providing an insightful, topical, and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. The editor and authors have produced an excellent book that will be extremely useful for all microbiologists.
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microbial quantification, target preparation, spin column, hydrolysis oligoprobes, fluorogenic chemistries, microbial template, fluorogenic oligoprobes, singleplex assays, routine clinical microbiology, hairpin oligoprobes, applications for routine laboratory testing, fluorescence melting curve analysis, duplex assay, amplicon strand, quasispecies variation, microbial diagnostics, amplicon accumulation, permitting fluorescence, quantitative molecular methods, external calibrators, bioterror agents, melting analysis, hydrolysis probe, internal amplification control, scorpion primers Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Routine Microbial Diagnostics, Rapid Detection of Bioterror Agents, Applied Biosystems, Astrid Petrich, Cell Probes, Aspects Med, Purification Kit, Roche Applied Science, New York, Texas Red, Nitsche Table, Bone Marrow Transplant, United States, Olfert Landt, West Nile, William Carman, Foster City, Green I-based, Mikael Kubista, Avoid G-rich, World Health Organization, Genome Res, Nick Saunders, Vendor Item Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Surprise Me!
microbial quantification, target preparation, spin column, hydrolysis oligoprobes, fluorogenic chemistries, microbial template, fluorogenic oligoprobes, singleplex assays, routine clinical microbiology, hairpin oligoprobes, applications for routine laboratory testing, fluorescence melting curve analysis, duplex assay, amplicon strand, quasispecies variation, microbial diagnostics, amplicon accumulation, permitting fluorescence, quantitative molecular methods, external calibrators, bioterror agents, melting analysis, hydrolysis probe, internal amplification control, scorpion primers Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Routine Microbial Diagnostics, Rapid Detection of Bioterror Agents, Applied Biosystems, Astrid Petrich, Cell Probes, Aspects Med, Purification Kit, Roche Applied Science, New York, Texas Red, Nitsche Table, Bone Marrow Transplant, United States, Olfert Landt, West Nile, William Carman, Foster City, Green I-based, Mikael Kubista, Avoid G-rich, World Health Organization, Genome Res, Nick Saunders, Vendor Item Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Surprise Me!
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