Receptor Signal Transduction Protocols (Methods in Molecular Biology) [Hardcover]

Gary B. Willars (Editor), R. A. John Challiss (Editor)

Book Description

Publication Date: March 1, 2004 | ISBN-10: 1588293297 | ISBN-13: 978-1588293299 | Edition: 2nd ed.

This new edition combines updates of key chapters from the first edition with a large number of new key methodologies that have emerged more recently for studying G protein-coupled receptors (GPCRs) and events immediately downstream of their activation. The methods are focused primarily on events at the receptor level, including ligand binding, the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors.

Editorial Reviews

Review

From reviews of the first edition...
"...should be present in all cellular and molecular laboratories of the world."
-Cellular and Molecular Biology

"...enthusiastically recommend[ed]...should figure prominently in any laboratory involved in receptor biochemistry, molecular biology, and pharmacology."
-Trends in Pharmacological Sciences

From the Back Cover

In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function.

Product Details

• Hardcover: 431 pages
• Publisher: Humana Press; 2nd ed. edition (March 1, 2004)
• Language: English
• ISBN-10: 1588293297
• ISBN-13: 978-1588293299
• Product Dimensions: 9.3 x 6.4 x 1.1 inches
• Shipping Weight: 1.8 pounds (View shipping rates and policies)
• Average Customer Review: 4.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #2,862,408 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

4.0 out of 5 stars Good laboratory manual on G protein-coupled receptor method, February 26, 2005

By 

Luis E. D. Gimenez (Nashville, TN. USA) - See all my reviews
(REAL NAME)   

This review is from: Receptor Signal Transduction Protocols (Methods in Molecular Biology) (Hardcover)

Laboratory manual edited by Gary B. Willars and R. A. John Chaliss. Maybe the most forthcoming limitation of this manual is it's "mission" to cover a vast array of protocols. This becomes a shortcoming because the space given for each technique and the theoretical backgrounds that substantiate them are very limited (short). Compare for instance the information on receptor binding given by Sebastian Lazareno on this book and the same author's chapter on the book edited by Tatsuya Haga. On the other hand, many of the articles are from experts in their fields and as an overall evaluation, the editors and contributors improved over the first edition witch was very limited in terms of coverage.