SUMO Protocols (Methods in Molecular Biology) [Hardcover]

Helle D. Ulrich (Editor)

Book Description

Publication Date: October 23, 2008 | ISBN-10: 1934115800 | ISBN-13: 978-1934115800 | Edition: 1

While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system.

Editorial Reviews

Review

From the reviews: "The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques … . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field … and regulated by SUMO. … for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves." (Peter O’Hare, Microbiology Today, July, 2009)

From the Back Cover

While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system.

Product Details

• Hardcover: 334 pages
• Publisher: Humana Press; 1 edition (October 23, 2008)
• Language: English
• ISBN-10: 1934115800
• ISBN-13: 978-1934115800
• Product Dimensions: 10.3 x 7.5 x 0.9 inches
• Shipping Weight: 1.8 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #3,223,530 in Books (See Top 100 in Books)