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DNA Sequencing (Laboratory Techniques in Biochemistry and Molecular Biology) (Vol 10)
  
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DNA Sequencing (Laboratory Techniques in Biochemistry and Molecular Biology) (Vol 10) [Paperback]

John Hindley (Author)


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Book Description

April 1, 1983 0444803858 978-0444803856 1
This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam-Gilbert chemical procedure; Computer methods for DNA sequencers; Appendices including contractions and special terms, cloning vectors, commercially available restriction endonucleases, and autoradiography.

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This book will be useful as an introduction and a starting point from which a serious student will follow the journals to keep up with the inevitable new developments -- - ANALYTICAL BIOCHEMISTRY

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Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
piperidine piperidine, ribosubstitution method, conformable tape, chain terminator sequencing, right contig, depurination analysis, primed synthesis methods, left contig, colourless plaques, sequencing channels, depurination products, contig lines, gel readings, descriptor lines, possible chain lengths, uncertainty codes, this contig, labelled end, blue marker dye, minus method, artifact bands, labelled fragments, manual agitation, annealing reaction, siliconized glass tube
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, New England Biolabs, Low Tris, Bethesda Research Laboratories, West Germany, Collaborative Research, Jesus Lane, Laboratory of Molecular Biology, Oxford Sampler, Fault Probable, Raven Scientific Ltd, Academic Press, Results Figure, Lyophilize Lyophilize Lyophilize Lyophilize, Edit Contig, Edit Position
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