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Short Protocols in Molecular Biology, 4th Edition [Paperback]

Frederick M. Ausubel (Editor), Roger Brent (Editor), Robert E. Kingston (Editor), David D. Moore (Editor), J. G. Seidman (Editor), John A. Smith (Editor), Kevin Struhl (Editor)
4.0 out of 5 stars  See all reviews (5 customer reviews)


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Short Protocols in Molecular Biology (2 volume set) Short Protocols in Molecular Biology (2 volume set) 4.0 out of 5 stars (5)
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Book Description

047132938X 978-0471329381 April 19, 1999 4
Short Protocols in Molecular Biology Fourth Edition The Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated-Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include:
* Informatics for Molecular Biologists
* Analysis of Protein Interactions
* Epitope Tagging
* Mathematics and Statistics for Molecular Biologists
Short Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods.


Editorial Reviews

Review

"Short Protocols is well constructed and very readable. It would be a useful addition to any research level library, but is especially recommended for libraries that have CPMB in reference as a good circulating copy." (E-Streams, Vol. 3, no. 1, January 2000)

From the Back Cover

Short Protocols in Molecular Biology Fourth Edition The Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated-Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include:
* Informatics for Molecular Biologists
* Analysis of Protein Interactions
* Epitope Tagging
* Mathematics and Statistics for Molecular Biologists
Short Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods.

Product Details

  • Paperback: 1104 pages
  • Publisher: Current Protocols; 4 edition (April 19, 1999)
  • Language: English
  • ISBN-10: 047132938X
  • ISBN-13: 978-0471329381
  • Product Dimensions: 10.9 x 8.7 x 3.1 inches
  • Shipping Weight: 7.8 pounds
  • Average Customer Review: 4.0 out of 5 stars  See all reviews (5 customer reviews)
  • Amazon Best Sellers Rank: #1,389,877 in Books (See Top 100 in Books)

 

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36 of 37 people found the following review helpful:
5.0 out of 5 stars A Must-Have for any Molecular Biologist..., March 17, 2000
By 
G (Northeast USA) - See all my reviews
This review is from: Short Protocols in Molecular Biology, 4th Edition (Paperback)
I originally bought this book as a secondary source of information (Maniatis has ben my bible now for a few years...) but as time has progressed I have found myself using this book more and more. The protocols are easy to follow, logically placed, give enough information so you understand what it is you are doing, are up to date, and are all in one volume. Yes, this has effectively replaced my copy of Molecular Cloning (which is in desperate need of an update). Great job!
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30 of 31 people found the following review helpful:
4.0 out of 5 stars A very good reference manual, May 26, 2000
By 
Math PC (Pasadena, CA, USA) - See all my reviews
This review is from: Short Protocols in Molecular Biology, 4th Edition (Paperback)
This book is an essential tool for people in the scientific field such as Molecular Biology (obviously), Biochemistry, and Neuroscience. It is comprehensive and up-to-date as far as the techniques are concerned. It is good value in a sense that you don't have to buy the whole "Current Protocols Series" which costs an arm and a leg if you do. Although nowadays, a lot of "kits" are commercially available, the techniques found in this book explain principles and provide different alternatives suited for your needs. Molecular Cloning by Maniatis et al., although needs updating, is still a helpful reference in my opinion and it complements this book.
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3 of 3 people found the following review helpful:
4.0 out of 5 stars The (little) Red book..., August 26, 2002
This review is from: Short Protocols in Molecular Biology, 4th Edition (Paperback)
Here is the little red bok.
If the big one is too expensive for you, you can always buy this. You'll find inside all the important protocols and data for molecular biology.It's up to date, and clearly presented.
Try it, and then buy the big one!
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Inside This Book (learn more)
First Sentence:
Mastery of current DNA technology requires familiarity with a small number of basic concepts and techniques. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
multiple gel caster, bead binding buffer, master dropout plate, cycles using primers, humidification optional, sieving agarose gel, infection cocktail, fusion protein solution, neurite outgrowth data, phosphate substrate buffer, detailed reaction conditions, complete spinner, alternate protocol, elegans clone, lambda dilution buffer, vaccinia virus stock, thioredoxin fusion proteins, microcentrifuge briefly, acid precipitation solution, canavanine plates, incubate until cells, pmol oligonucleotide primer, dropout plates, serial dilution analysis, trypsinized virus
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Additional Materials, Amersham Pharmacia Biotech, Life Technologies, Boehringer Mannheim, New England Biolabs, Roger Brent, Invitrogen Human, Kevin Struhl, Stanley Tabor, Apr Contains, Clontech Human, Vector Laboratories, Gel Bond, Victoria Lundblad, Beckman Coulter, Sepharose Fast Flow, Becton Dickinson, Blue Dextran, International Biotechnologies, Kmr Same, Perkin-Elmer Applied Biosystems, Sun Microsystems, World Wide Web, Blast Hits, Cmr Same
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