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Short Protocols in Molecular Biology (2 volume set) [Paperback]

Frederick M. Ausubel (Editor), Roger Brent (Editor), Robert E. Kingston (Editor), David D. Moore (Editor), J. G. Seidman (Editor), John A. Smith (Editor), Kevin Struhl (Editor)
4.0 out of 5 stars  See all reviews (5 customer reviews)


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Book Description

0471250929 978-0471250920 November 5, 2002 5th Edition, 2 Volume Set
Expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations.

The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research

Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level.


Editorial Reviews

Review

"Short Protocols is well constructed and very readable. It would be a useful addition to any research level library, but is especially recommended for libraries that have CPMB in reference as a good circulating copy." (E-Streams, Vol. 3, no. 1, January 2000) --This text refers to an out of print or unavailable edition of this title.

From the Back Cover

Short Protocols in Molecular Biology Fourth Edition The Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated-Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include:
* Informatics for Molecular Biologists
* Analysis of Protein Interactions
* Epitope Tagging
* Mathematics and Statistics for Molecular Biologists
Short Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods. --This text refers to an out of print or unavailable edition of this title.

Product Details

  • Paperback: 1512 pages
  • Publisher: Current Protocols; 5th Edition, 2 Volume Set edition (November 5, 2002)
  • Language: English
  • ISBN-10: 0471250929
  • ISBN-13: 978-0471250920
  • Product Dimensions: 11.1 x 8.6 x 2.9 inches
  • Shipping Weight: 7.6 pounds
  • Average Customer Review: 4.0 out of 5 stars  See all reviews (5 customer reviews)
  • Amazon Best Sellers Rank: #415,206 in Books (See Top 100 in Books)

 

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36 of 37 people found the following review helpful:
5.0 out of 5 stars A Must-Have for any Molecular Biologist..., March 17, 2000
By 
G (Northeast USA) - See all my reviews
I originally bought this book as a secondary source of information (Maniatis has ben my bible now for a few years...) but as time has progressed I have found myself using this book more and more. The protocols are easy to follow, logically placed, give enough information so you understand what it is you are doing, are up to date, and are all in one volume. Yes, this has effectively replaced my copy of Molecular Cloning (which is in desperate need of an update). Great job!
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30 of 31 people found the following review helpful:
4.0 out of 5 stars A very good reference manual, May 26, 2000
By 
Math PC (Pasadena, CA, USA) - See all my reviews
This book is an essential tool for people in the scientific field such as Molecular Biology (obviously), Biochemistry, and Neuroscience. It is comprehensive and up-to-date as far as the techniques are concerned. It is good value in a sense that you don't have to buy the whole "Current Protocols Series" which costs an arm and a leg if you do. Although nowadays, a lot of "kits" are commercially available, the techniques found in this book explain principles and provide different alternatives suited for your needs. Molecular Cloning by Maniatis et al., although needs updating, is still a helpful reference in my opinion and it complements this book.
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3 of 3 people found the following review helpful:
4.0 out of 5 stars The (little) Red book..., August 26, 2002
Here is the little red bok.
If the big one is too expensive for you, you can always buy this. You'll find inside all the important protocols and data for molecular biology.It's up to date, and clearly presented.
Try it, and then buy the big one!
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Inside This Book (learn more)
First Sentence:
Mastery of current DNA technology requires familiarity with a small number of basic concepts and techniques. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
multiple gel caster, polyvinylidene wrap, lambda top agar, cycles using primers, phosphate substrate buffer, lambda dilution buffer, pulse labeling medium, acid precipitation solution, detergent stock solutions, alternate protocol, glycerol storage buffer, sample loop size, infection cocktail, nondenaturing lysis buffer, serial dilution analysis, column wash solution, standard protein solution, nucleotide stock solutions, cationic lipid reagent, guanidinium solution, immunized species, lambda plates, microcentrifuge briefly, basic protocol, random octamers
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Short Protocols, Support Protocol, Additional Materials, Amersham Pharmacia Biotech, Life Technologies, Problem Possible, Boehringer Mannheim, Kevin Struhl, New England Biolabs, Stanley Tabor, Gel Bond, Applied Biosystems, Construction of Recombinant, Beckman Coulter, Chromatographic Conditions Column, Interactions Page, Libraries Page, Becton Dickinson, Chloroform Isoamyl, Molecular Probes, Sequencing Page, Bacteriophages Page, Bio-Rad Mini-Protean, International Biotechnologies, Oak Ridge
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