The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of RNA probes for ISHH. These interesting chapters describe the preparation of different radiolabeled probes for ISHH. They also discuss their respective advantages and limitations, and describe current results based on the use of these various probes. Sections of the text highlight low and high resolution autoradiography for ISHH, the use of biotin-labeled probes for ISHH, as well as the use of ISHH in combination with established anatomical techniques. In Situ Hybridization Histochemistry answers all of your questions regarding the quantification of ISHH. It also provides a practical description of typical protocols, both from molecular biology and histology. Investigators will understand and value this useful, powerful tool-whatever their backgrounds might be.
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First Sentence:
Prior to the development of in situ hybridization histochemistry, pedigree analysis or somatic cell genetics was required to estimate the location of genes on chromosomes. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
mouse cerebellar mutants, developmental myelination, mouse neurological mutants, hiotinylated probes, situ transcription, reeler mutation, exposed silver grains, demyelinated lesions, nonradioactive probes, transcription procedure, myelin basic protein gene, hybridization histochemistry, nerve growth factor synthesis, probe binding, adult cerebellum, white matter regions, subbed slides, transcription technique, cellular resolution, synthetic oligonucleotide probes, spinal cord sections, hybridization mix, granule cells, transcription signal, mouse cerebellum Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Brain Res, Acids Res, Cell Biol, Oxford University Press, Unaffected Intrinsic, Cerebellum Purkinje, General Considerations, Academic Press, Cell Res, Cerebellum Bergmann, Cerebellum Granule, Bruin Res New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Prior to the development of in situ hybridization histochemistry, pedigree analysis or somatic cell genetics was required to estimate the location of genes on chromosomes. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
mouse cerebellar mutants, developmental myelination, mouse neurological mutants, hiotinylated probes, situ transcription, reeler mutation, exposed silver grains, demyelinated lesions, nonradioactive probes, transcription procedure, myelin basic protein gene, hybridization histochemistry, nerve growth factor synthesis, probe binding, adult cerebellum, white matter regions, subbed slides, transcription technique, cellular resolution, synthetic oligonucleotide probes, spinal cord sections, hybridization mix, granule cells, transcription signal, mouse cerebellum Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Brain Res, Acids Res, Cell Biol, Oxford University Press, Unaffected Intrinsic, Cerebellum Purkinje, General Considerations, Academic Press, Cell Res, Cerebellum Bergmann, Cerebellum Granule, Bruin Res New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 7
books:
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7
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- In Situ Hybridization (Current Topics in Microbiology and Immunology) by Ashley T. Haase on page 36, page 162, and page 165
- Techniques of Autoradiography by Andrew W. Rogers on page 188
- The Rat Nervous System, Third Edition (Paxinos, The Rat Nervous System) by George Paxinos on page 106
- Multiple Sclerosis by Jürg Kesselring on page 67
- In Situ Hybridization: Applications to Neurobiology by Karen L. Valentino on page 162
See all 7 books this book cites
- Visualization of Nucleic Acids by Gerard Morel on page 211
- Molecular Regulation of Arousal States (Cellular and Molecular Neuropharmacology Series) by Ralph Lydic on page 89
- In Situ Hybridization Protocols (Methods in Molecular Biology) by K. H. Andy Choo on page 311
- Cystic Fibrosis: Methods and Protocols (Methods in Molecular Medicine) by William R. Skach on page 214
- Receptor Localization: Laboratory Methods and Procedures by Marjorie Ariano on page 140
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