In situ hybridization is a proven, powerful technique with applications in chromosome and genome analysis, as well as gene expression. Covering a carefully selected range of techniques with immediate and general applications in research and clinical diagnosis, the book starts with genome and DNA mapping, continues through gene expression localization in wholemount and tissue sections, and on to ultrastructural levels. The step-by-step protocols used reflect research in these areas and are all reproducible.
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2 of 2 people found the following review helpful:
4.0 out of 5 stars
Good general text.,
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This review is from: In Situ Hybridization: Laboratory Companion (Hardcover)
Clearly explains basic in situ hybridization methods for various types of tissues and nucleic acid detection. Covers most detection and labelling methods simply and consisely. Useful as a first stop on the road to learning about in situ hybridization.
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Inside This Book
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First Sentence:
Genomic in situ hybridization is a method involving hybridization of labelled total genomic DNA, used as a probe, to spread or sectioned denatured chromosome preparations in situ, followed by detection and visualization of the sites of hybridization. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
antifade solution containing propidium iodide, constitutional mosaicism, genomic probing, blocking powder, dense fibrillar component, nucleolar components, situ labelling, numerical chromosome aberrations, tumor clone, labelled nucleotide, stringent temperature, metaphase chromosome spreads, centromeric probes, hybridization mixture, gene repeat, sheep serum, blocking solution, probe amount, interphase cytogenetics, structural chromosome aberrations, zebrafish embryos, nucleotide mix, formamide concentration, chromosome painting, metaphase cells Key Phrases - Capitalized Phrases (CAPs): (learn more)
Boehringer Mannheim, Cell Genet, Texas Red, Hum Genet, Theor Appl Genet, Mal Gen Genet, Vector Laboratories, Vector Labs, Vector Red, Cancer Genet, Cell Sci, Ann Bot, Practical Approach New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Genomic in situ hybridization is a method involving hybridization of labelled total genomic DNA, used as a probe, to spread or sectioned denatured chromosome preparations in situ, followed by detection and visualization of the sites of hybridization. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
antifade solution containing propidium iodide, constitutional mosaicism, genomic probing, blocking powder, dense fibrillar component, nucleolar components, situ labelling, numerical chromosome aberrations, tumor clone, labelled nucleotide, stringent temperature, metaphase chromosome spreads, centromeric probes, hybridization mixture, gene repeat, sheep serum, blocking solution, probe amount, interphase cytogenetics, structural chromosome aberrations, zebrafish embryos, nucleotide mix, formamide concentration, chromosome painting, metaphase cells Key Phrases - Capitalized Phrases (CAPs): (learn more)
Boehringer Mannheim, Cell Genet, Texas Red, Hum Genet, Theor Appl Genet, Mal Gen Genet, Vector Laboratories, Vector Labs, Vector Red, Cancer Genet, Cell Sci, Ann Bot, Practical Approach New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 6
books:
See all 6 books this book cites
- In Situ by Henry W. Hodges in Front Matter, and page 65
- Cell Biology (Molecular and Cell Biochemistry Series) by C. A. Smith on page 121
- Animal Reproduction: Invited Papers (Beltsville Symposia in Agricultural Research, Vol. 3) by H.W. Hawk in Front Matter
- Cell Biology by Ricki Lewis on page 121
- Clinical Medicine: with STUDENT CONSULT Access by Parveen Kumar CBE BSc MD FRCP FRCP(Edin) in Front Matter
See all 6 books this book cites
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