In Situ Hybridization Techniques for the Brain (IBRO Handbook Series: Methods in the Neurosciences) [Paperback]

Z. Henderson (Editor)

Book Description

ISBN-10: 047196140X | ISBN-13: 978-0471961406 | Publication Date: November 1996

Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.

Editorial Reviews

From the Publisher

This book shows researchers in neuroscience how to detect and identify specific genes in brain tissue and brain cells. It provides a comprehensive listing of protocols and includes the theoretical background to in situ hybridization in addition to specific technical details. Step-by-step instructions enable readers to plan and carry out procedures in their own laboratories. Covers everything from basic lab setup to sophisticated lab work. Includes details on everyday procedures, recent developments, and interpreting data.

Product Details

• Paperback: 176 pages
• Publisher: John Wiley & Sons (November 1996)
• Language: English
• ISBN-10: 047196140X
• ISBN-13: 978-0471961406
• Product Dimensions: 9.7 x 6.9 x 0.4 inches
• Shipping Weight: 12 ounces
• Amazon Best Sellers Rank: #9,970,990 in Books (See Top 100 in Books)