John R. Chapman recreates the success of his earlier acclaimed book (Protein and Peptide Analysis by Mass Spectrometry, 1996) with a major new collection of cutting-edge methods in the analysis of proteins and peptides. Contributed by well-established investigators, each chapter provides background information before guiding the researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. The applications covered range widely and include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing. Authoritative and eminently practical, Mass Spectrometry of Proteins and Peptides demonstrates the full scope of this versatile analytical technique. All protein chemists and biochemists, as well as bioanalytical scientists, who want to exploit these powerful methods in their work, will find this book indispensable.
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Editorial Reviews
Review
"John Chapman has followed on from his excellent earlier volume 'Protein and Peptide Analysis by Mass Spectrometry' by bringing together in this both very readable and informative volume 27 individual chapters from acknowledged practitioners of the application of mass spectrometry to peptides and proteins. Each chapter is packed with real information both for the biologist and mass spectroscopist and contains a wealth of detail on sample reparation and the subsequent interpretation of the MS results. ...The 'hot' topics of the investigation of non-covalent complexes, protein-protein interactions and higher order protein structures by mass spectrometry are addressed in separate chapters. Any laboratory working on peptides and proteins whether they use MS or not should have access to this book. It is a goldmine."-European Peptide Society Newsletter
"...a well crafted book using the experience of respected pioneers of modern mass spectrometric methods....Many of the methods used in proteomic studies rely on mass spectrometric analysis of proteins and peptides, as well as protein separation technology. The latter is dealt with to some extent by this volume; however, the mass spectrometric aspects are very well covered....This volume shows the great versatility of mass spectrometric analysis, in this case related to protein and peptides, indicating that most analytical challenges can be met by the technique, especially when combined with innovative sample preparation. For the clinical biochemist with an interest in proteins and peptides, this volume is an important reference. For other biochemists it represents a view of a great potential, which will develop as our use of the human genome information progresses."-Annals of Clinical Biochemistry
"The availability of a compilation of authoritative reviews that include a wealth of practical detail regarding sample preparation and presentation as well as instrument operation is both timely and of considerable value. In total there are some twenty-seven comprehensive descriptions of mass spectrometric analyses of proteins, peptides and mixtures of various complexity and provenance. The elucidation of higher-order structure is described as well as primary sequence determination, epitope mapping and cell typing using several substrates from highly refined isolates to more complex mixtures and whole cells. The contributions are provided by over sixty well established researchers and include detailed information for sample isolation and preparation as well as a comprehensive bibliography on each topic area. Further information is collated into a series of appendices covering an abbreviation glossary, listings of mass/abundance values for elements, amino acids and monosaccharide residues and a literature update on protein mass spectrometry for 1997 - 1998. This volume is a valuable addition to the series: it is comprehensive in terms of the range of applications considered and extensive in the descriptions of the analytical process. It is unusual among reviews of mass spectrometric applications for the wealth of experimental detail provided an this aspect alone makes it an essential item for every biological mass spectrometry and proteomics laboratory." - Chromatographia
"...a well crafted book using the experience of respected pioneers of modern mass spectrometric methods....Many of the methods used in proteomic studies rely on mass spectrometric analysis of proteins and peptides, as well as protein separation technology. The latter is dealt with to some extent by this volume; however, the mass spectrometric aspects are very well covered....This volume shows the great versatility of mass spectrometric analysis, in this case related to protein and peptides, indicating that most analytical challenges can be met by the technique, especially when combined with innovative sample preparation. For the clinical biochemist with an interest in proteins and peptides, this volume is an important reference. For other biochemists it represents a view of a great potential, which will develop as our use of the human genome information progresses."-Annals of Clinical Biochemistry
"The availability of a compilation of authoritative reviews that include a wealth of practical detail regarding sample preparation and presentation as well as instrument operation is both timely and of considerable value. In total there are some twenty-seven comprehensive descriptions of mass spectrometric analyses of proteins, peptides and mixtures of various complexity and provenance. The elucidation of higher-order structure is described as well as primary sequence determination, epitope mapping and cell typing using several substrates from highly refined isolates to more complex mixtures and whole cells. The contributions are provided by over sixty well established researchers and include detailed information for sample isolation and preparation as well as a comprehensive bibliography on each topic area. Further information is collated into a series of appendices covering an abbreviation glossary, listings of mass/abundance values for elements, amino acids and monosaccharide residues and a literature update on protein mass spectrometry for 1997 - 1998. This volume is a valuable addition to the series: it is comprehensive in terms of the range of applications considered and extensive in the descriptions of the analytical process. It is unusual among reviews of mass spectrometric applications for the wealth of experimental detail provided an this aspect alone makes it an essential item for every biological mass spectrometry and proteomics laboratory." - Chromatographia
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Inside This Book
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Key Phrases - Statistically Improbable Phrases (SIPs):
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sample preparation techniques, searching sequence databases, active site residues, epitope mapping, protein glycosylation, higher order protein structure, nitrosylated peptides, spin gel pieces, mannose transporter complex, kinase preparation incubated, isonicotinic acid methyl ester, epitope excision, neat formic acid, signature peptides, venom samples, naja kaouthia, protein chemical modification, mass calibrant, diammonium hydrogen citrate, long neurotoxin, affinity mass spectrometry, oat avenins, prompt fragmentation, accessory files, crude venoms Key Phrases - Capitalized Phrases (CAPs): (learn more)
Mass Spectrom, Rapid Commun, Protein Sci, Molecular Biology, Humana Press Inc, Boehringer Mannheim, Protein Chem, Trends Biochem, New York, Ion Proc, Rapid Identification of Bacteria, Analysis of S-Nitrosohemoglobin, Nucleic Acids Res, Matrix Preparations, Palo Alto, Peptide Sequencing, Degradation of Peptides, Toxin Mass Fingerprints of Crude Venoms, Protein G-Plus, Protein-Protein Interactions, Biological Fluids, On-Target Reactions, Methods Enzymol, Multiple Component Monitoring Paradigm, Applied Biosystems New!
Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
sample preparation techniques, searching sequence databases, active site residues, epitope mapping, protein glycosylation, higher order protein structure, nitrosylated peptides, spin gel pieces, mannose transporter complex, kinase preparation incubated, isonicotinic acid methyl ester, epitope excision, neat formic acid, signature peptides, venom samples, naja kaouthia, protein chemical modification, mass calibrant, diammonium hydrogen citrate, long neurotoxin, affinity mass spectrometry, oat avenins, prompt fragmentation, accessory files, crude venoms Key Phrases - Capitalized Phrases (CAPs): (learn more)
Mass Spectrom, Rapid Commun, Protein Sci, Molecular Biology, Humana Press Inc, Boehringer Mannheim, Protein Chem, Trends Biochem, New York, Ion Proc, Rapid Identification of Bacteria, Analysis of S-Nitrosohemoglobin, Nucleic Acids Res, Matrix Preparations, Palo Alto, Peptide Sequencing, Degradation of Peptides, Toxin Mass Fingerprints of Crude Venoms, Protein G-Plus, Protein-Protein Interactions, Biological Fluids, On-Target Reactions, Methods Enzymol, Multiple Component Monitoring Paradigm, Applied Biosystems New!
Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
29
books
cite this book:
See all 29 books citing this book
- Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research by Jerzy Silberring on page 99
- Biostatistical Methods (Methods in Molecular Biology) by Stephen W. Looney in Front Matter
- Protein Kinase Protocols (Methods in Molecular Biology) by Alastair D. Reith in Front Matter
- DNA Topoisomerase Protocols, Part II: Enzymology & Drugs (Methods in Molecular Biology) (v. 2) by Neil Osheroff in Front Matter
- Analytical Techniques for Biopharmaceutical Development by Roberto Rodriguez-Diaz on page 273
See all 29 books citing this book
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