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Gene Therapy Protocols (Methods in Molecular Medicine)
 
 
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Gene Therapy Protocols (Methods in Molecular Medicine) [Paperback]

Jeffrey R. Morgan (Editor)

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Book Description

October 2001 0896038696 978-0896038691 2nd
In this fully updated and revised 2nd edition of Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step laboratory procedures that enable its successful therapeutic application.

Editorial Reviews

Review

From Reviews of the First Edition:
"...extremely impressive...a must, even for researchers who are already familiar with gene transfer techniques..." -Molecular Medicine Today

"...succeeds admirably..."-Genetical Research

From Reviews of the Second Edition:

"Each protocol is written in a standard format, consisting of introduction, materials, methods, notes, and references. The introductions vary in length and detail. Many of them are illustrated with tables, charts, schematics, and/or photographs. The materials and methods sections are quite detailed, and there are many notes clarifying steps or outlining potential problems. Gene Therapy Protocols is recommended for university, hospital, and corporate libraries serving researchers who are working on gene therapies." - E-STREAMS

"All the chapters are well written, and the protocols are easy to understand. This book is applicable to those directly involved in the field of gene therapy." - Pharmaceutical Research

"...a valuable resource for background information in the field of gene transfer vector development, as well as providing concise and clearly written protocols for the production of these vectors." - Molecular Biotechnology

"Each of the 32 chapters is constructed like a cooking-book with detailed instructions and illustrations of how to perform the procedures. For anyone who is planning or is already practically involved in gene therapy experiments, this impressive book is a must in the laboratory." - Acta Derm Venereol

"Second edition of the "Gene Therapy Protocols" in successful series of books "Methods in Molecular Medicine" from Humana Press, is certainly a publication, which appeared in the right time. The comprehensibility of the book represents great value also for researcher. This collection of gene therapy protocols is a valuable and indispensable resource for basic scientists, graduate students, and postdoctoral fellows. Even clinical researchers will find the book very informative and useful. The book represents an outstanding work, which should not be missed in all laboratories dealing with gene therapy." - Neoplasma

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Inside This Book (learn more)
First Sentence:
Nonviral gene delivery has great potential for replacement of recombinant protein therapy. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
producer plasmid, photochemical transfection, bilateral tibialis anterior muscles, modified skin substitutes, tumor growth media, amplicon virions, photochemical internalization, foamy virus type, priming medium, bioartificial muscles, citric saline, host cell biology, hybrid amplicon vectors, iodixanol solution, tas gene, stable packaging cell lines, mouse muscle cells, complementing cell line, acellular dermis, gene gun device, producer cell lines, vector rescue, foamy virus vectors, vivo electroporation, human adenovirus vectors
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Gene Ther, Molecular Medicine, Humana Press Inc, Applied Biosystems, Nucleic Acids Res, Cancer Res, Cell Biol, New York, Cold Spring Harbor Laboratory Press, San Diego, American Type Culture Collection, Crude Lysate, Gene Med, Preparation of High-Titer Viral Stocks, Del Tatto, Generation of Recombinant Virus, Collaborative Biomedical Products, Generation of F-Defective, Microbix Biosystems, National Institutes of Health, Oak Ridge, Phoenix Pharmaceuticals, Ann Arbor, Avanti Polar Lipids, Beckman Ultra-Clear
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