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Tissue In Situ Hybridization: Methods in Animal Development (Embo Practical Course)
 
 
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Tissue In Situ Hybridization: Methods in Animal Development (Embo Practical Course) [Paperback]

Trevor Jowett (Author)


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Book Description

0471164038 978-0471164036 January 1997
TISSUE IN SITU HYBRIDIZATION

Methods in Animal Development

Trevor Jowett

The European Molecular Biology Organization (EMBO) course on tissue in situ hybridization in animal developmental biology has served as an important and highly respected forum for the latest advances in the methodology of this valuable research tool. Developed from EMBO course materials, Tissue

In Situ Hybridization provides scientists and researchers worldwide with detailed coverage of new approaches, techniques, and protocols, along with up-to-date information on more established procedures.

Focusing particularly on the two-color in situ hybridization method to whole-mount embryos and tissue sections, this practical resource also compares different methods of producing differentially colored signals, and includes the results of protocol experiments with fluorescent and other alternative in situ hybridization techniques. Special features include:

  • Photographic examples—including color plates—to complement the text
  • Clear explanations of the principles underlying different methods
  • Detailed discussion and comparison of the different methods
  • Valuable troubleshooting advice and practical guidance
  • Comprehensive index, allowing quick and easy access to specific topics

Compiled by a leading expert in the field, Tissue In Situ Hybridization is an indispensable asset to professionals and researchers working in the areas of developmental, cell, and molecular biology.


Editorial Reviews

From the Publisher

Based on a EMBO practical course for those wishing to apply the method of in situ hybridization to their research in developmental biology. The main emphasis is on the two-color in situ hybridization method to whole-mount embryos and tissue sections, and it also compares the different methods of producing differentially colored signals. It also includes the results of protocol experiments with fluorescent and other alternative in situ hybridization techniques.

From the Back Cover

TISSUE IN SITU HYBRIDIZATION

Methods in Animal Development

Trevor Jowett

The European Molecular Biology Organization (EMBO) course on tissue in situ hybridization in animal developmental biology has served as an important and highly respected forum for the latest advances in the methodology of this valuable research tool. Developed from EMBO course materials, Tissue

In Situ Hybridization provides scientists and researchers worldwide with detailed coverage of new approaches, techniques, and protocols, along with up-to-date information on more established procedures.

Focusing particularly on the two-color in situ hybridization method to whole-mount embryos and tissue sections, this practical resource also compares different methods of producing differentially colored signals, and includes the results of protocol experiments with fluorescent and other alternative in situ hybridization techniques. Special features include:

  • Photographic examples—including color plates—to complement the text
  • Clear explanations of the principles underlying different methods
  • Detailed discussion and comparison of the different methods
  • Valuable troubleshooting advice and practical guidance
  • Comprehensive index, allowing quick and easy access to specific topics

Compiled by a leading expert in the field, Tissue In Situ Hybridization is an indispensable asset to professionals and researchers working in the areas of developmental, cell, and molecular biology.


Product Details

  • Paperback: 132 pages
  • Publisher: Wiley (January 1997)
  • Language: English
  • ISBN-10: 0471164038
  • ISBN-13: 978-0471164036
  • Product Dimensions: 10.1 x 7.2 x 0.8 inches
  • Shipping Weight: 11.5 ounces
  • Amazon Best Sellers Rank: #5,347,118 in Books (See Top 100 in Books)

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Inside This Book (learn more)
First Sentence:
Techniques for the localization of mRNAs in situ were first developed in the late 1970s and are now widely used in many areas of biological research. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
zebrafish probed, contaminated glassware soaked, monitor the staining reaction, sequential alkaline phosphatase, embryo probed, blocking powder, rhodamine filter set, sheep serum, zebrafish embryos, fluorescein probe, antidigoxigenin antibody, posthybridization washes, blocking solution, larger embryos, mount embryos, staining buffer, hybridization buffer, tail protein, first incubated, nonspecific binding sites, staining solution, alkaline phosphatase substrate, blue precipitate, probe synthesis, hybridization solution
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Vector Red, Vector Labs, Color Figure Section, Molecular Probes, Internet Web, Sigma Fast, Boehringer Mannheim, Boehringer Blocking, Adobe Photoshop, Agar Scientific, Vector Laboratories, Mannheim Fab, Sequential Incubation, Vector Elite
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