Trace Quantitative Analysis by Mass Spectrometry [Hardcover]

Robert K. Boyd (Author), Cecilia Basic (Author), Robert A. Bethem (Author)

Book Description

ISBN-10: 0470057718 | ISBN-13: 978-0470057711 | Publication Date: June 11, 2008 | Edition: 1

This book provides a serious introduction to the subject of mass spectrometry, providing the reader with the tools and information to be well prepared to perform such demanding work in a real-life laboratory. This essential tool bridges several subjects and many disciplines including pharmaceutical, environmental and biomedical analysis that are utilizing mass spectrometry:

• Covers all aspects of the use of mass spectrometry for quantitation purposes
• Written in textbook style to facilitate understanding of this topic
• Presents fundamentals and real-world examples in a ‘learning-though-doing’ style

Editorial Reviews

Review

"This work, well documented and easy to read, will without doubt delight teachers and researchers in analytical chemistry, permitting them to choose apparatus best suited to their analytical problems, to understand how to use them, and finally to put them to work in developing and validating quantitative methods in thev laboratory." (l'actualité chimique, October 2010)"There are many textbooks on the general principles and applications of mass spectrometry, but none of them cover these topics in such detail as the present book. … A valuable contribution to the mass spectrometry textbooks that are currently available, and is useful for both the neophyte and the specialist." (Analytical and Bioanalytical Chemistry, July 2009)

"I highly recommend this book … to all those readers of this journal involved in quantitative analysis … Boyd, Basic, and Bethem provided us with a wealth of easy accessible information on this subject." (Journal of Statistical Software, January 2009)

"Trace Quantitative Analysis by Mass Spectrometry is a wonderful book for beginner and intermediate students, analysts, and scientists keen on learning more about mass spectrometry. The authors' discussion of history and the evolution of relevant science make the text lively and engaging, no small achievement for a book about analytical science." (PharmTech.com, December 2, 2008)

From the Back Cover

This book is devoted to the use of mass spectrometry in quantitative measurements of amounts of target (known) chemical compounds present at trace levels in complex matrices, such as drugs and their metabolites in body fluids, pesticide residues in foodstuffs, contaminants in drinking water, etc. Such demanding measurements, defined as 1 part in 10⁶ – 10¹², involve the use of a wide range of apparatus and of experimental procedures and methods of data evaluation, all of which must be utilized properly if reliable estimates of chemical concentrations and their associated uncertainties are to be obtained. While this is true of any chemical analysis, modern advances in trace-level analysis are critically dependent on developments in mass spectrometry.

The approach adopted throughout the book is to emphasize the fundamentals underlying the scientific instruments and methodologies, illustrated by historically important developments and more recent innovations. However, discussions of the fundamentals are reinforced and related to the real-world by two chapters dedicated to method development and validation. Finally, how "the fundamental things apply" to real-world problems is illustrated in the final chapter devoted to representative examples from a wide range of application areas.

This book does not cover important branches of mass spectrometry that provide accurate and precise quantitative measurements of relative concentrations, e.g. variations in isotopic ratios of an element by isotope ratio mass spectrometry and accelerator mass spectrometry.  Rather, it is mainly concerned with determinations of absolute amount of substance, particularly for small organic molecules present at trace levels in complex matrices.

The book

• covers analysis of "small" (< 2000 Da) organic molecules, in environmental and biomedical matrices (trace level analyses of metals and other elements are not included).

• provides a comprehensive introduction to the ancillary techniques and tools, including statistical analysis, that must be coordinated to provide a reliable result for a trace-level quantitative analysis by mass spectrometry.
• discusses method development and validation, GLP, etc., in the context of a fitness-for-purpose approach that is applicable to any quantitative analytical method.
• written at a level that presupposes some basic undergraduate-level knowledge of chemistry, physics, and mathematics and statistics.
• treats the more recent developments in quantitative analysis of specific proteins in biological systems using proteolytic peptides as surrogate analytes.

Any book such as this can only be regarded as a preparation for the real learning process in this demanding practical art, namely "learning by doing" i.e. working on real-life problems in a laboratory. However, this book will be useful both in providing enough background information (“learning from others”) that the first exposure to the "learning by doing" process will not seem quite so daunting, and also will provide useful background for evaluation of new technologies as they appear.

Product Details

• Hardcover: 748 pages
• Publisher: Wiley; 1 edition (June 11, 2008)
• Language: English
• ISBN-10: 0470057718
• ISBN-13: 978-0470057711
• Product Dimensions: 9.8 x 7.7 x 1.8 inches
• Shipping Weight: 3.9 pounds (View shipping rates and policies)
• Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #163,714 in Books (See Top 100 in Books)
  • #51 in Books > Professional & Technical > Professional Science > Chemistry > Analytic

More About the Author

Discover books, learn about writers, read author blogs, and more.

Customer Reviews

Most Helpful Customer Reviews

3 of 3 people found the following review helpful:

5.0 out of 5 stars The Greatest Book ever created :-), May 22, 2009

By 

Charlie (NY, USA) - See all my reviews

Amazon Verified Purchase

This review is from: Trace Quantitative Analysis by Mass Spectrometry (Hardcover)

I initially bought this book for further information on one chapter. But this book is so in-depth yet concise, I may make it through, cover to cover. The title does not do it justice. It covers all the aspects of Mass Spectrometry that people tend to forget about - theory, principles, classical techniques, chromatography, calibration, the list goes on, and it refers the reader to other sections for background information. Definitely a must for anyone interested in doing quantitative mass spec. Two thumbs up.