After a review of the state-of-the-art in the RNA polymerase II transcription field, subsequent chapters present a systematic guide to the investigation of regulatory mechanisms for newly identified genes, and discuss approaches for synthesizing recombinant transcription factors, analyzing their binding, and deciphering their mode of action. Carey (UCLA) and Smale (Howard Huges Medical Institute and UCLA) write for graduate students, physician-scientists, and others, either beginning or already established, who are undertaking in-depth studies in the analysis of gene expression in model systems or disease states. They put methods in the context of underlying theory, recommend experimental strategies, encourage creativity in investigative design, and explain protocols, with troubleshooting advice.
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Book Description
Editorial Reviews
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"Carey and Smale have undertaken the heroic task of assembling a compendium of Concepts, Strategies and Techniques for analyzing..." -- Howard Hues Medical Institute, U.C. Berkley
"In keeping with the fact that gene expression in eukaryotes is regulated primarily at the level of transcription..." -- Rockefeller University
"In keeping with the fact that gene expression in eukaryotes is regulated primarily at the level of transcription..." -- Rockefeller University
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Most Helpful Customer Reviews
1 of 1 people found the following review helpful:
5.0 out of 5 stars
a great reference for transcription lab work!,
By TsunamiMommy (Washington DC) - See all my reviews
This review is from: Transcriptional Regulation in Eukaryotes: Concepts, Strategies and Techniques (Hardcover)
I purchased this book for my lab and I cannot praise it highly enough. The only problem is that grad students and postdocs keep stealing it from my office and I'm always having to go out and hunt it down on someone's bench! When introducing a new technique to your lab, it is a good idea to read the chapater on that technique before you pick up your pipet. The descriptions of methodology and the degree of detail are first rate. I hope that Carey and Smale bring out a new edition soon that covers the new innovations in the transcription field from the five years since their current edition was published.Lori Bernstein Assistant Professor Texas A & M University
3 of 7 people found the following review helpful:
4.0 out of 5 stars
Good Book for the beginner and expert....,
By micro94@eudoramail.com (SEOUL) - See all my reviews
This review is from: Transcriptional Regulation in Eukaryotes: Concepts, Strategies and Techniques (Hardcover)
There are so many method for experiment regarding transcriptional regulations. In this book, you may find brief purpose, background knowledge and technical guide for the allmost experiment about transcriptional regulations.
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Inside This Book
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First Sentence:
One of the central goals of the gene expression field is understanding how a mammalian organism regulates transcription of approximately 100,000 genes in the proper spatial and temporal patterns. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
comprehensive mutant analysis, distant control regions, defined control element, anticipated start site, ethylation interference assay, stable transfection assay, transcription complex assembly, gene regulation analysis, permanganate probing, standard reporter gene, coactivator fraction, relevant control elements, important control regions, protein purification strategy, appropriate functional assay, ethylated phosphates, reporter templates, multiprotein family, yeast holoenzyme, stable assay, synergistic transcription, important control elements, expression library screen, promoter escape, relevant activator Key Phrases - Capitalized Phrases (CAPs): (learn more)
Genes Dev, New York, Cold Spring Harbor, Methods Enzymol, Nucleic Acids Res, Cell Press, Cell Biol, Roche Molecular Biochemicals, Oxford University Press, Amersham Pharmacia Biotech, Genes Deu, United Kingdom, Invitrogen Corp, Life Technologies, Saran Wrap, Blom van Assendelft, Current Opinion, Methods Mol, Copyright Academic Press Ltd, Trends Biochem, Trends Genet, Michael Haykinson, National Academy of Sciences, Santa Cruz Biotechnology, Van Dyke New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
One of the central goals of the gene expression field is understanding how a mammalian organism regulates transcription of approximately 100,000 genes in the proper spatial and temporal patterns. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
comprehensive mutant analysis, distant control regions, defined control element, anticipated start site, ethylation interference assay, stable transfection assay, transcription complex assembly, gene regulation analysis, permanganate probing, standard reporter gene, coactivator fraction, relevant control elements, important control regions, protein purification strategy, appropriate functional assay, ethylated phosphates, reporter templates, multiprotein family, yeast holoenzyme, stable assay, synergistic transcription, important control elements, expression library screen, promoter escape, relevant activator Key Phrases - Capitalized Phrases (CAPs): (learn more)
Genes Dev, New York, Cold Spring Harbor, Methods Enzymol, Nucleic Acids Res, Cell Press, Cell Biol, Roche Molecular Biochemicals, Oxford University Press, Amersham Pharmacia Biotech, Genes Deu, United Kingdom, Invitrogen Corp, Life Technologies, Saran Wrap, Blom van Assendelft, Current Opinion, Methods Mol, Copyright Academic Press Ltd, Trends Biochem, Trends Genet, Michael Haykinson, National Academy of Sciences, Santa Cruz Biotechnology, Van Dyke New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 2
books:
1
book
cites this book:
- Current Protocols in Molecular Biology by Frederick M. Ausubel on page 367, page 383, and page 384
- Molecular Cell Biology by Harvey Lodish on page 298
- The Biochemistry of Cell Signalling by Ernst J. M. Helmreich on page 186
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