Unraveling DNA: Molecular Biology for the Laboratory [Paperback]

Michael R. Winfrey (Author), Marc A. Rott (Author), Alan Wortman (Author)

Book Description

Publication Date: February 1, 1997 | ISBN-10: 0132700344 | ISBN-13: 978-0132700344 | Edition: 1

This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.

Editorial Reviews

From the Publisher

This innovative manual introduces students to all of the basic techniques of modern molecular biology using an integrated series of laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. By organizing the exercises as part of a major cloning project, students get the sense of performing a complete cloning project, rather than just learning a collection of procedures. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. Collectively, the entire series of exercises requires approximately three quarters to one full semester to complete with two laboratory periods per week. Smaller portions of the manuals are easily adapted to fewer lab periods.

From the Back Cover

This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.

Product Details

• Paperback: 400 pages
• Publisher: Benjamin Cummings; 1 edition (February 1, 1997)
• Language: English
• ISBN-10: 0132700344
• ISBN-13: 978-0132700344
• Product Dimensions: 10.9 x 8.5 x 0.7 inches
• Shipping Weight: 1.7 pounds (View shipping rates and policies)
• Average Customer Review: 4.3 out of 5 stars  See all reviews (3 customer reviews)
• Amazon Best Sellers Rank: #720,438 in Books (See Top 100 in Books)

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Customer Reviews

Most Helpful Customer Reviews

3 of 3 people found the following review helpful:

3.0 out of 5 stars This book badly needs revision, March 19, 2002

By 

Antoinette (Bethesda MD) - See all my reviews

This review is from: Unraveling DNA: Molecular Biology for the Laboratory (Paperback)

The material in the introductory text preceeding most of the exercizes lacks clarity, is poorly written and sadly confuses the reader. The introduction to the lux operon seems to be a very good example to illustrate the point. This book was made possible by a grant from the NSF and if the authors continue to market this book without revision it would be just be a travesty and an insult to intelligence. Please take note of the shortcomings of this book before decinding to buy it.

5.0 out of 5 stars Two thumbs up, March 13, 2002

By A Customer

This review is from: Unraveling DNA: Molecular Biology for the Laboratory (Paperback)

One of the best lab texts around. Provides a thorough exposure to the most commonly used molecular laboratory techniques. Highly recommended for faculty, who focus on creating a hands-on learning environment.

5.0 out of 5 stars Wonderful Lab Manual, February 26, 2001

By 

Gobind Swamy (Wilmington, DE United States) - See all my reviews

This review is from: Unraveling DNA: Molecular Biology for the Laboratory (Paperback)

This is a wonderful text clear and concise, it explores the bulk of the basic lab techniques and procedures in molecular biology. This highly readable text builds on the cumulative experiences of some highly skilled academicians and researchers. Highly recommended for any molecular biology lab course or as a useful reference.