Using Antibodies: A Laboratory Manual [Spiral-bound]

Ed Harlow (Author), David Lane (Author)

Book Description

ISBN-10: 0879695447 | ISBN-13: 978-0879695446 | Publication Date: December 1, 1998

Few technical manuals have become standards in biomedicine. Antibodies: A Laboratory Manual, by Ed Harlow and David Lane, has had that status for a decade. Now there is a new and even higher standard Using Antibodies: A Laboratory Manual. Harlow and Lane have completely revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been entirely rewritten, reorganized, and updated to provide background, context, and step-by-step instructions for techniques that range from choosing the right antibody and handling it correctly, to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included. Rather than presenting an array of solutions for working with antibodies and antigens, Using Antibodies instead identifies in each case the best approach to specific problems. These recommendations include more detail in the protocols, extensive advice on avoiding and solving problems, information regarding proper controls, and extensive illustration of theory, methods, and results, both good and bad. An additional bonus included with this manual is a set of Portable Protocols, step-by-step instructions for the most frequently used and essential techniques printed on spill-proof, durable cards that can be annotated and used directly at the bench. The expert advice in Using Antibodies is presented using an imaginative design with extensive use of color and graphic elements calculated to help readers plan and execute their experiments efficiently and accurately. A newly available type of binding will maintain the manual's integrity during years of use. Related Titles from the Publisher Using Antibodies: Portable Protocols (Additional Sets) Antibodies: A Laboratory Manual

Product Details

• Spiral-bound: 495 pages
• Publisher: Cold Spring Harbor Laboratory Press (December 1, 1998)
• Language: English
• ISBN-10: 0879695447
• ISBN-13: 978-0879695446
• Product Dimensions: 12 x 9.3 x 1.5 inches
• Shipping Weight: 4.7 pounds
• Average Customer Review: 3.0 out of 5 stars  See all reviews (2 customer reviews)
• Amazon Best Sellers Rank: #1,648,671 in Books (See Top 100 in Books)

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Customer Reviews

Most Helpful Customer Reviews

10 of 10 people found the following review helpful:

1.0 out of 5 stars Should be [less money], Not [more money]!, October 20, 2002

By 

James j Hathcock (New York) - See all my reviews

This review is from: Using Antibodies: A Laboratory Manual (Spiral-bound)

Although I liked the outdated 1988 version of this book, this one is extremely simplistic and omits much of the previously included material. Most of the information included can be found on the web and is shallow compared to Current Protocols or other texts. Although it serves as a reasonable introduction for novices to this area it's extremely overpriced for [money]!!!

I would reccomend Monoclonal Antibodies by Goding ([money]),

2 of 3 people found the following review helpful:

5.0 out of 5 stars Two Thumbs Up, October 24, 2000

By A Customer

This review is from: Using Antibodies: A Laboratory Manual (Spiral-bound)

This is an exceptional reference for anyone using abtibodies in the laboratory. It is a *must-have* for anyone venturing into unknown teritory. For the experienced individual forced to bang out new protocols with a cranky antibody, this reference is priceless.