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Working With DNA (The Basics) Paperback – April 30, 2007

ISBN-13: 978-0415374644 ISBN-10: 0415374642 Edition: 1st

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Product Details

  • Paperback: 414 pages
  • Publisher: Taylor & Francis; 1st edition (April 30, 2007)
  • Language: English
  • ISBN-10: 0415374642
  • ISBN-13: 978-0415374644
  • Product Dimensions: 1 x 6.8 x 9.5 inches
  • Shipping Weight: 1.5 pounds (View shipping rates and policies)
  • Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Best Sellers Rank: #2,571,435 in Books (See Top 100 in Books)

Editorial Reviews

Review

"…there are problem based exercises, with the answers at the end, which could be valuable for tutorials, before practical classes or just to check that your research students are understanding the techniques that they are going to use in your laboratory." Journal of Biological Education

About the Author

Stan Metzenberg, Associate Professor, Department of Biology, California State University at Northridge

Stan Metzenberg obtained his Ph.D. degree in Molecular Biology at the University of Wisconsin-Madison, and was a post-doctoral fellow at the University of California-San Francisco.  He is currently an Associate Professor of Biology at California State University, Northridge, where he teaches courses in biotechnology and recombinant DNA techniques.  His principal research interests are in the molecular biology of infectious diseases.


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Most Helpful Customer Reviews

1 of 1 people found the following review helpful By C. Cloninger on May 4, 2008
Format: Paperback
I must confess that I am a student of Dr. Metzenberg's and Recombinant DNA Technology is one of the more difficult classes I have taken.

This book is informative in a way that many text books are not; it tells you both information about the subject as well as general instructions on how to do some important procedures. Most importantly, it tells you WHY you must do certain steps in protocols, and the advantages and disadvantages of different approaches. It also discusses the method of using Klenow to shape DNA fragments as opposed to the more popular method of using restriction enzymes, which is a lovely bit of knowledge to have.

"Working With DNA" was helpful with the background knowledge, although in the laboratory and in an exam setting, nothing beats hands on experience. The practice problems help with this, although the one problem and answer explanation that I wish was included would be an example of the PCR based 4 primer method of making internal mutations. This is an easy concept to grasp, but a very difficult task to actually carry out correctly on pen and paper. A written step by step specific example using actual base pairs instead of the more general explanation would be helpful.

Overall, I enjoyed the book and will keep it for handy reference while working in the lab.
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