Thin-Layer Chromatography Supplies
Thin-layer chromatography (TLC) is a method to separate chemical compounds for analysis or purification. In a thin-layer chromatography lab experiment or process, adsorbent material such as silica gel is spread on a flat TLC plate, and a drop of the chemical compound is applied at the base of the plate with a dropper or pipette. The plate is placed into a developing tank that contains a solvent. The solvent travels up the plate by capillary action. Some parts of the sample compound are attracted to the stationary silica, and bind to it, while others continue to travel with the solvent. To visualize the separated components on the plate, a chemical reagent is applied to the plate with a sprayer. Enhanced methods of TLC include high-performance TLC (HPTLC), which automates parts of the process, including sample application, development, and detection, providing increased resolution and accuracy.