Practical HPLC Method Development, 2nd Edition (Hardcover)

~ (Author), Joseph J. Kirkland (Author), Joseph L. Glajch (Author) "Every day many chromatographers face the need to develop a high-performance liquid chromatography (HPLC) separation..." (more)
Key Phrases: critical band pair, benzoic acid sample, initial gradient run, New York, Academic Press, San Diego (more...)

Editorial Reviews

Product Description

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

From the Publisher

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Product Details

• Hardcover: 765 pages
• Publisher: Wiley-Interscience; 2nd edition (March 3, 1997)
• Language: English
• ISBN-10: 047100703X
• ISBN-13: 978-0471007036
• Product Dimensions: 9.3 x 6.4 x 1.7 inches
• Shipping Weight: 2.9 pounds (View shipping rates and policies)
• Average Customer Review: 4.7 out of 5 stars  See all reviews (6 customer reviews)
• Amazon.com Sales Rank: #345,994 in Books (See Bestsellers in Books)

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Customer Reviews

Most Helpful Customer Reviews

11 of 11 people found the following review helpful:

5.0 out of 5 stars A great book - information overload, July 15, 2002

By	"gand30" (Plainsboro, NJ) - See all my reviews

This is a very useful reference to have if you work extensively on HPLC. I find its of more use to understand concepts ...there is a lot of information here.

Which means - if you are looking for a solution to a problem and you want to research separation theory - a great book.

If your looking for a quick fix to an HPLC problem - not as good. This is a detailed, extensive and well written text on HPLC Method development - wish there was more on validation here though.

9 of 10 people found the following review helpful:

5.0 out of 5 stars Plactical and Rational method Development!, March 14, 2000

By	Makoto Niwa (Tokyo, Japan) - See all my reviews

Rational Method development for HPLC is resented. Instruction of rational development of 3-solvent separation system is excellent. This book also has useful know-hows and tools in practical laboratory experiments, such as strength of hydrogen bonding of base silica and nomograph for estimation of "solvent strength" for reversed phase HPLC.

5.0 out of 5 stars Best book on development of HPLC separations, November 24, 2008

By	Norman Strojny "retired tech-person" (western desert of Utah) - See all my reviews (REAL NAME)

This is the best book of which I know, on the development of HPLC (High Performance Liquid Chromatographic) separations.

However, there have been important changes in HPLC since this edition, especially with smaller diameter particles for packing materials.

I wish Wiley would prod the authors on an update.