High-speed countercurrent chromatography, a technique used to separate substances into their individual components, was first developed in the late 1970s when it overshadowed other methods of chromatography with its superior capacity to achieve rapid and efficient separation. This newer system is now employed in a wide range of applications, most notably for extracting medicinal drugs from plants or purifying dyes.
High-Speed Countercurrent Chromatography is the first book to provide a comprehensive and up-to-date treatment of this technique. It covers all the latest developments in equipment, theory, and applications, as well as many topics not previously published anywhere, such as the purification of recombinant proteins directly from a crude E. coli lysate, the development of instruments that produce highly concentrated pure fractions, and successful CCC/MS interfacing.
Charting the remarkable progress high-speed CCC has made over the past five years, the book discusses the method's advantages over other forms of chromatography and shows how this versatile system permits the separations chemist to impose a number of variations upon the fundamental chromatographic process. The authors review a multitude of practical details involved in various procedures and manipulations, from dual CCC to hyphenated techniques. Finally, the book covers virtually all the fields in which CCC is particularly advantageous, including the extraction and/or purification of natural products, marine products, antibiotics, hormones, medicinal herbs, dyes, proteins and peptides, and inorganic materials such as rare earths.
This book is both a practical guide for analytical chemists and lab workers, and a valuable reference for students taking courses in separation methods at the graduate level. It also opens a window on future developments in this rapidly advancing field.
HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
What every analytical chemist needs to know about this important new technique
High-Speed Countercurrent Chromatography is the first book to be devoted entirely to this popular and fast-developing technique for separation and purification. It covers areas of particular interest to chemists who deal with both natural products and synthetic organic substances, and it is also extremely useful for those studying structure activity relationships.
Assembled by well-known authorities in the field, this book:
- Presents both theory and practice of high-speed CCC
- Brings together information that has previously been scattered throughout journal articles, as well as information not previously published anywhere
- Provides a handy and time-saving reference on the use of CCC, specifying a variety of processes and separation methods
- Describes all the latest developments in the field, including state-of-the-art instrumentation and various applications
- Offers numerous examples, especially from pharmaceutical applications, throughout the text
- Reviews all the areas in which CCC has provided special advantages, such as the extraction of medicinal drugs from plants or purifying dyes
For professional chemists and researchers in the pharmaceutical and medical industries, as well as cosmetics, agriculture, and other industrial and commercial pursuits, this book is an excellent practical guide, a helpful and easily accessible reference, and a watershed of ideas for further research and future applications.
From the Publisher
This contributed text describes the remarkable progress that has been made in the high speed CCC technique including purification of recombinant proteins directly from a crude E. coli lysate, development of pH-peak-focusing and pH-zone-refining CCC to produce highly concentrated pure fractions comparable to displacement chromatography and successful CCC/MS interfacing. Contains a number of recent applications such as separation and purification of natural, marine and medicinal products, antibiotics, dyes and inorganic elements. Introduces the unique use of form CCC and liquid-liquid dual CCC.
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