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Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology)
 
 
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Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology) (Hardcover)

by Gary D. Foster (Editor), Sally Taylor (Editor) "Methods developed for plant virology have been of central importance to other branches of plant pathology..." (more)
Key Phrases: sonchus yellow net virus, superinfecting virus, inoculation buffer, Humana Press Inc, Plant Virology Protocols, Transgenic Resistance Edited (more...)
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Product Description
A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.

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Inside This Book (learn more)
First Sentence:
Methods developed for plant virology have been of central importance to other branches of plant pathology. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
sonchus yellow net virus, superinfecting virus, inoculation buffer, laboratory sealing film, barley mild mosaic virus, genomic blot analysis, alfalfa mosaic virus coat protein, aucuba mosaic virus, capsid protein sequence, local lesion assay, triple gene block, sucrose pad, mosaic virus genome, plant reoviruses, pea early browning virus, virus coat protein gene, virus purification, plant rhabdoviruses, density gradient fractionator, virus pellet, mannitol buffer, mosaic potexvirus, plant virus diseases, tobacco streak virus, grinding buffer
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Plant Virology Protocols, Transgenic Resistance Edited, Nucleic Acids Res, New York, Plant-Microbe Interact, United Kingdom, Plant Pathol, Boehringer Mannheim, Plant Mol, Taxonomy of Viruses, Boca Raton, Cold Spring Harbor Laboratory, Plant Physiol, Plant Rhabdovirus Purification, Computer Analysis of Amino Acid Sequences, Methods Enzymol, Molecular Analysis of Transgenic Rice, Virus Res, San Diego, Easy Hyb, Plant Mot, Production of Transgenic Rice, Russet Burbank, Association of Applied Biologists
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