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Cystic Fibrosis: Methods and Protocols (Methods in Molecular Medicine)
 
 
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Cystic Fibrosis: Methods and Protocols (Methods in Molecular Medicine) (Hardcover)

by William R. Skach (Editor) "Mutation detection in an integral part of disease diagnosis and patient study..." (more)
Key Phrases: pairwise binding assay, rotational mucus transport, agarose bead model, Humana Press Inc, Cell Biol, Gene Ther (more...)
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Editorial Reviews

Review
"The coverage of methodologies is extremely broad and will be useful for those wanting to find out more about some of the less routine techniques used in the analysis of the CFTR protein/chloride channel. The style of protocol-based chapters enables in manuscripts due to considerations of space or potential repetition of previously published techniques. As such many of the chapters are valuable." -Molecular Biotechnology "Undoubtedly this book will be regarded as essential reading for scientists in the field. It provides details of research methodology, which are rarely published in peer reviewed journals. It will also enable researchers to identify subtle differences in techniques in their own laboratories from those described in these chapters. As such, it will be a very important book for all those who conduct laboratory-based research into cystic fibrosis." -Journal of Paediatrics and Child Health

Product Description
Cystic Fibrosis: Methods and Protocols consolidates cutting-edge in vitro, cellular, and whole animal laboratory protocols into an indispensable resource. From electrophysiology and cell biology, to animal models and gene therapy, this comprehensive set of methods provides the step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Specific protocols describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems to determine how mutations subvert CFTR function, and in vivo protocols to examine how CFTR dysfunction produce multisystem pathology in both human and animal models.

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Inside This Book (learn more)
First Sentence:
Mutation detection in an integral part of disease diagnosis and patient study. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
pairwise binding assay, rotational mucus transport, agarose bead model, tip feeding needle, wash apical, calibrator diluent, chamber halves, chronic pseudomonas infection, human bronchial xenografts, cell surface biotinylation, halide indicators, apical membrane resistance, human airway ion transport, set the optical density, pipet resistance, heating manifold, conformational maturation, dropout plates, transmembrane conductance regulator chloride channel, airway fluid, phosphorimage analysis, microdissecting forceps, bait protein, transport phenotype, airway surface liquid
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Cell Biol, Gene Ther, Texas Red, New York, Care Med, Boehringer Mannheim, Cystic Fibrosis Foundation, Axon Instruments, Pflugers Arch, Amersham Pharmacia Biotech, Rockefeller University Press, San Diego, Academic Press, Cell Mol, National Institutes of Health, Cell Physiol, Nucleic Acids Res, Fisher Scientific, Journal of General Physiology, Molecular Probes, Sigma Chemical, World Precision Instruments, Avanti Polar Lipids, Jet Plus
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