Protein Crystallization (Iul Biotechnology Series) (Hardcover)

by Terese M. Bergfors (Editor) "The first protein crystals, of hemoglobin, were grown over 150 years ago and were immediately recognized as an exceptional and valuable discovery..." (more)
Key Phrases: imidazole malate, epitaxial jumps, microbatch trials, Hampton Research, Acta Cryst, Mol Biol (more...)

Editorial Reviews

Review
This is an essential handbook for anyone engaged in crystallization of macromolecules. It is exceptionally well organized and illustrated and has contributions from all the leaders in what continues to be a challenging and critically important field. --Dr. William L. Duax, American Crystallographic Association, Chief Executive Officer

Why is crystallization important? At different times, different aspects of protein crystallography have assumed the role of bottleneck. Thus at one time the bottleneck consisted of the lack of a methodology for structure determination, even if a sufficient number of diffraction intensitities were available for structure solution. With the advent of powerful methods for structure solution, the bottleneck shifted to the difficulty in obtaining crystals in sufficient number and of suitable quality to do the diffraction experiment. The present book is dedicated to the problem of describing current methods for protein crystallization and describing them as clear laboratory protocols. --Dr. Herbert A. Hauptman, Nobel Laureate in Chemistry --This text refers to the Hardcover edition.

Product Description
Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization. Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Thirty full-color plates for evaluating crystallization drops. Separate section of laboratory exercises, ideal for crystallization courses. A-Z glossary. --This text refers to the Hardcover edition.

Product Details

• Hardcover: 306 pages
• Publisher: International University Line; 1 edition (February 1, 1999)
• Language: English
• ISBN-10: 0963681753
• ISBN-13: 978-0963681751
• Product Dimensions: 9.1 x 6.2 x 1.1 inches
• Shipping Weight: 1.4 pounds
• Amazon.com Sales Rank: #1,325,378 in Books (See Bestsellers in Books)