![@b-Cyclodextrin-hexamethylene diisocyanate copolymer-coated zirconia for separation of racemic 2,4-dinitrophenyl amino acids in reversed-phase liquid chromatography ... [An article from: Analytica Chimica Acta]](http://ecx.images-amazon.com/images/I/415FBN4EPVL._SL500_AA240_.jpg)
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
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We report use of @b-cyclodextrin (CD)-hexamethylene diisocyanate (HMDI) copolymer(CDPU)-coated zirconia (CDPUZ) as a chiral stationary phase for separation of enantiomers of a set of 2,4-dinitrophenyl (DNP) amino acids in reversed-phase liquid chromatography. The effects of polymer loading on zirconia and HMDI/CD molar ratio in the preparation of CDPU on retention and selectivity in the separation of DNP-amino acids in were examined to find optimum polymer loading and HMDI/CD ratio. It was observed that 8% loading of the CDPU prepared with the HMDI/CD molar ratio of 4 gave the best separation of the amino acids investigated. Retention and chiral selectivity of CDPUZ were also compared to those for previously reported carboxymethyl-@b-CD-coated zirconia (CMCDZ). CDPUZ gave better chiral separation than CMCDZ for the DNP-amino acids.
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