Virus Separation and Purification Methods 0th Edition

by Polson (Author), Alfred Polson (Other Contributor)

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ISBN-13: 978-0824791490

ISBN-10: 0824791495

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This reference presents many contributions made by Dr Alfred Polson during his 41 years of research into the physicochemical properties of plant and animal viruses - detailing his timesaving approaches to the characterization, extraction, separation, concentration and purification of viruses, proteins, antibodies and biopolymers.;Describing successful laboratory techniques featuring the Beckman preparative centrifuge, this reference: examines the electro-extraction process for isolating the maximum amount of viruses from infected plant material; analyzes zone electrophoresis in sugar concentration gradients as a powerful tool for purifying entero-, insect born and insect viruses; contains material on the use of a reorienting gradient centrifuge rotor to separate components of the haemolymph of the mollusc Turbo sarmaticus as a model for virus separation; explains the construction of a modified thin-layer ultracentrifuge rotor for concentrating viruses into suspension rather than pellets; elucidates the use of inserts in ultracentrifuge tubes in order to decrease the time of centrifuging; and introduces a method for determining pure viruse suspension densities.;This resource is intended for microbiologists, virologists, biochemists, molecular and cell biologists, immunologists, biochemical and bioprocess engineers, chemical engineers and graduate students in these disciplines.