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Product Description
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods.
Key Features
* Only volume on Green Fluorescent Protein research
* Covers all aspects of GFP
* Provides specific microscopic and data analysis methods
* Discusses the design and construction of GFP fusion proteins
* Covers GFP expression in animals, insects, plants, and microbes
* Details procedures for time lapse imaging of living cells
* Explains how to implement single molecule fluorescence detection with GFP
* Discusses dual label GFP strategies for multicolor fluorescence
* Presents fluorescence resonance energy transfer methods with GFPs
* Details quantitative fluorescence imaging techniques
* Extensively illustrated with color photographs
Book Info
The Scripps Research Institute, La Jolla, CA. Contains a set of protocols for working with green fluorescent proteins (GFP) at several levels. For researchers. Plastic-comb binding, hardcover also available.
See all Editorial Reviews
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods.
Key Features
* Only volume on Green Fluorescent Protein research
* Covers all aspects of GFP
* Provides specific microscopic and data analysis methods
* Discusses the design and construction of GFP fusion proteins
* Covers GFP expression in animals, insects, plants, and microbes
* Details procedures for time lapse imaging of living cells
* Explains how to implement single molecule fluorescence detection with GFP
* Discusses dual label GFP strategies for multicolor fluorescence
* Presents fluorescence resonance energy transfer methods with GFPs
* Details quantitative fluorescence imaging techniques
* Extensively illustrated with color photographs
Book Info
The Scripps Research Institute, La Jolla, CA. Contains a set of protocols for working with green fluorescent proteins (GFP) at several levels. For researchers. Plastic-comb binding, hardcover also available.
See all Editorial Reviews
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Inside This Book Citations: This book cites 39 books Explore: Citations | Books on Related Topics | Concordance | Text Stats Key Phrases - SIPs: centromere dynamics, lac operator repeats, individual centromeres, centrosome dynamics, cardiomyocyte transfected (more) Key Phrases - CAPs: Cell Biol, New York, Cold Spring Harbor, Sorting of Cells Based, Adobe Photoshop (more) Browse Sample Pages: Front Cover | Copyright | Table of Contents | Excerpt | Index | Back Cover | Surprise Me! |
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Inside This Book
(learn more)
First Sentence:
Green bioluminescence has been known as a feature of marine coelenterates for many years, but only in 1971 with the discovery by Morin and Hastings (1971a) did it become known that the green color derives from an intrinsically green fluorescent protein. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
centromere dynamics, lac operator repeats, individual centromeres, centrosome dynamics, cardiomyocyte transfected, fluorophore saturation, bleached strip, aequorea green fluorescent protein, victoria green fluorescent protein, actin patches, bleaching intensity, different optical sections, posttranslational autoxidation, embryonic cardiomyocytes, gfp variants, microtubule motor protein, spindle dynamics, mean pixel values,
Green bioluminescence has been known as a feature of marine coelenterates for many years, but only in 1971 with the discovery by Morin and Hastings (1971a) did it become known that the green color derives from an intrinsically green fluorescent protein. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
centromere dynamics, lac operator repeats, individual centromeres, centrosome dynamics, cardiomyocyte transfected, fluorophore saturation, bleached strip, aequorea green fluorescent protein, victoria green fluorescent protein, actin patches, bleaching intensity, different optical sections, posttranslational autoxidation, embryonic cardiomyocytes, gfp variants, microtubule motor protein, spindle dynamics, mean pixel values,