by Benjamin Lewin
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by Robert D. Goldman
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by Bruce Alberts
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by Uri Alon
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Protein Localization by Fluorescence Microscopy
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Editorial Reviews
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" ... a useful bencjh-aid. The Editor-Victoria Allan - ans all of the contributors should be congratulated on putting together a valuable reference text " Trends Cell Biology
Product Description
There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques-- including live cell work--against the potential pitfalls, offering invaluable "tricks of the trade" along the way.
See all Editorial Reviews
" ... a useful bencjh-aid. The Editor-Victoria Allan - ans all of the contributors should be congratulated on putting together a valuable reference text " Trends Cell Biology
Product Description
There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques-- including live cell work--against the potential pitfalls, offering invaluable "tricks of the trade" along the way.
See all Editorial Reviews
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First Sentence:
Immunofluorescence on fixed samples forms a vital part of most investigations into protein function, since it provides an idea of the whereabouts of a protein within the cell, which in turn can give important clues as to the protein's function. Read the first page Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, Academic Press, Molecular Probes, San Diego, Cell Sci, Roche Diagnostics, New York, Plant Journal, Santa Cruz, Cold Spring Harbor Laboratory Press, Journal of Microscopy, Natl Acad, Practical Approach, Agar Scientific Ltd, Continued Method, Laboratory Manual, Oxford University Press, Texas Red, Laboratory Handbook, Plenum Press, Stuart Scientific, Applied Precision Inc, Biomedicals Inc, Current Biology, Silicon Graphics New!
Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Immunofluorescence on fixed samples forms a vital part of most investigations into protein function, since it provides an idea of the whereabouts of a protein within the cell, which in turn can give important clues as to the protein's function. Read the first page Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, Academic Press, Molecular Probes, San Diego, Cell Sci, Roche Diagnostics, New York, Plant Journal, Santa Cruz, Cold Spring Harbor Laboratory Press, Journal of Microscopy, Natl Acad, Practical Approach, Agar Scientific Ltd, Continued Method, Laboratory Manual, Oxford University Press, Texas Red, Laboratory Handbook, Plenum Press, Stuart Scientific, Applied Precision Inc, Biomedicals Inc, Current Biology, Silicon Graphics New!
Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 27
books:
See all 27 books this book cites
2
books
cite this book:
- Cell Biology (Molecular and Cell Biochemistry Series) by C. A. Smith on page 132, and page 161
- Handbook of Biological Confocal Microscopy by James B. Pawley on page 25, and page 107
- Fine Structure Immunocytochemistry by Gareth Griffiths on page 26, and page 47
- Antibodies: A Laboratory Manual by Ed Harlow on page 25, and page 205
- Instant Notes in Molecular Biology (Bios Instant Notes) by P. Turner on page 108
See all 27 books this book cites
- Cartilage and Osteoarthritis (Methods in Molecular Medicine) by Frédéric De Ceuninck on page 125
- Transgenic Mouse Methods and Protocols (Methods in Molecular Biology) by Marten H. Hofker on page 143
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