by James B. Pawley
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Basic Methods in Microscopy: Protocols and Concepts from Cells: A Laboratory Manual by David L. Spector |
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Digital Microscopy, Volume 81, Third Edition: Methods in Cell Biology by Greenfield Sluder |
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Editorial Reviews
Review
Overall, this new book is a superb resource for imaging techniques commonly used to improve time-lapse data, and a valuable addition to any laboratory's library. It emphasizes the multitude of model systems that can be studied using time-lapse imaging and will surely motivate many more to join the live cell imaging revolution. Nature Cell Biology The book has brought together some of the leading experts in the field of live cell imaging, each contributing a chapter that covers their particular area of expertise...Each chapter clearly introduces its subject, outlines the methods available (some with detailed protocols), and, most importantly, describes the pitfalls that can be encountered during live cell imaging. The length and detail contained is, in my opinion, ideal for gaining a good grounding in the subject of interest. I feel that this will be an excellent resource for experienced microscopists attempting live cell imaging for the first time. It would also be ideal for students wishing to learn more about the important functional information that can be gained from imaging living cells. The Quarterly Review of Biology Live Cell Imaging: A Laboratory Manual, edited by Goldman and Spector, is an extremely useful addition to their previous book Cells: A Laboratory Manual. As its name implies, it is a laboratory manual and as such contains all the pertinent information to carry out the full range of live cell imaging techniques, but it also has enough relevant background to be a useful reference manual or text for an advanced teaching course. Not only that, it provides the necessary theory of how the various optical systems that are available work, which are most suited to what task, and in many cases provides useful practical tips from some of the best practitioners in the field on how to modify and adapt these systems to best suit individual needs. The text is littered with examples, often including comparative studies to highlight the advantages of one technique or piece of equipment over another. The book comes with a digital versatile disk (DVD) containing movies to further illustrate the techniques discussed. The DVD provides extremely useful examples of what can be achieved, but also serves to highlight the realistic quality of information that can be gained by any given technique, something the na ef;ve reader may not be fully aware of. Laboratory Animals
--This text refers to the
Paperback
edition.
Product Description
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book.
The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual, are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
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Inside This Book
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First Sentence:
THE 1990s BROUGHT AN ABUNDANCE OF TECHNICAL ADVANCES that enabled both the widespread use of molecular cloning techniques and the measurement of cellular processes in living systems using optical microscopy. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
lambda stack, acquisition photobleaching, universal compensator, photobleaching reversibility, cell imaging experiments, traditional polarizing microscope, live cell imaging, fluorescent speckle microscopy, polarization optical train, egg salts solution, lac operator repeats, bleaching mode, caged phosphopeptides, micropipette fabrication, live cell experiments, living endothelial cells, bleach iterations, emission cross talk, pipette storage container, chemical fluorophores, retardance values, caged peptides, confinement tree, live cell microscopy, prebleach images Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, New York, Cold Spring Harbor, Cancer Res, Cell Sci, Academic Press, Nucleic Acids Res, Plenum Press, World Precision Instruments, Methods Enzymol, National Institutes of Health, New Jersey, Column Buffer, San Diego, Alexa Fluor, Cautions Appendix, Applied Precision, Biosciences Clontech, Optical Microscopy Primer, Plan Apochromat, Cell Motil, Chroma Technology, Controlling Acquisition Photobleaching, John Wiley, Oxford University Press New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
THE 1990s BROUGHT AN ABUNDANCE OF TECHNICAL ADVANCES that enabled both the widespread use of molecular cloning techniques and the measurement of cellular processes in living systems using optical microscopy. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
lambda stack, acquisition photobleaching, universal compensator, photobleaching reversibility, cell imaging experiments, traditional polarizing microscope, live cell imaging, fluorescent speckle microscopy, polarization optical train, egg salts solution, lac operator repeats, bleaching mode, caged phosphopeptides, micropipette fabrication, live cell experiments, living endothelial cells, bleach iterations, emission cross talk, pipette storage container, chemical fluorophores, retardance values, caged peptides, confinement tree, live cell microscopy, prebleach images Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, New York, Cold Spring Harbor, Cancer Res, Cell Sci, Academic Press, Nucleic Acids Res, Plenum Press, World Precision Instruments, Methods Enzymol, National Institutes of Health, New Jersey, Column Buffer, San Diego, Alexa Fluor, Cautions Appendix, Applied Precision, Biosciences Clontech, Optical Microscopy Primer, Plan Apochromat, Cell Motil, Chroma Technology, Controlling Acquisition Photobleaching, John Wiley, Oxford University Press New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 8
books:
See all 8 books this book cites
1
book
cites this book:
- Live Cell Imaging: A Laboratory Manual by Robert D. Goldman in Front Matter, and page 353
- Caged Compounds, Volume 291 (Methods in Enzymology) by John N. Abelson on page 157
- Cells : A Laboratory Manual (Volume 1`) Culture and Biochemical Analysis of Cells by David L. Spector on page 353
- Instant Notes in Molecular Biology (Bios Instant Notes) by P. Turner on page 370
- Nucleic Acids (Methods in Molecular Biology) by John M. Walker on page 473
See all 8 books this book cites
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