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Green Fluorescent Protein: Applications & Protocols (Methods in Molecular Biology)
 
 
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Green Fluorescent Protein: Applications & Protocols (Methods in Molecular Biology) (Hardcover)

~ Barry W. Hicks (Editor) "Recently, we cloned six new green flourescent protein (GFP)-like flourescent proteins from five species of Antozoa (1), including one red-emitting variant (DsRed), which is now..." (more)
Key Phrases: color representation please, table microcentrifuge, permuted proteins, Life Technologies, Humana Press Inc, Cell Biol (more...)
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Editorial Reviews

Review

"A particularly helpful adjunct is provided by a CD-ROM included with the book. This disc contains color images for many of the protocols and is especially useful for visualization. The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value." -Doody's Health Sciences Book Review Journal

"...this book truly is a collection of "Applications and protocols" and such is the degree of detail in the methods described that it should perhaps have been sub-titled "A Laboratory Manual." -The Analyst


Product Description

Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses. A valuable companion CD-ROM displays many color figures and videos from over half the chapters.

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Inside This Book (learn more)
First Sentence:
Recently, we cloned six new green flourescent protein (GFP)-like flourescent proteins from five species of Antozoa (1), including one red-emitting variant (DsRed), which is now commercially available. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
color representation please, table microcentrifuge, permuted proteins, urea induction, transduction media, permuted variants, new termini, circular gene, retroviral packaging cell lines, retroviral producer cells, nucleolar fluorescence, cocoon layer, harvest media, fluorescent colonies, cyan fluorescent protein, packaging cells, green fluorescent protein, spectral overlap integral, phosphorylation sequence, transduced cells, gfp reporter gene, retroviral constructs, staining buffer, viral supernatant, circular permutation
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Life Technologies, Humana Press Inc, Cell Biol, Acids Res, Cold Spring Harbor, New York, New England Biolabs, Applications Using, Lipofectin Reagent, Cancer Res, Nucleocytoplasmic Transport Using, Palo Alto, Becton Dickinson, Hirschie Johnson, Transgenic Bovine Embryo Selection, Biosensor Substrates, Boehringer Mannheim, American Type Culture Collection, Cell Res, Drug-Induced Translocation of Proteins, Fluobody Immunosensors, Gene Ther, Mechanisms of Protein Trafficking, National Institutes of Health, Nikon Diaphot
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