This scanner has a single USB 2.0 plug to a Micro USB plug cable. Although there is usually more than one USB port available to use on your PC, there is only one Micro USB plug output on the scanner. I am sure that there is either a splitter cable, or a USB Hub that can take the signal from the scanner and send it to two different USB 2.0 ports on two separate PC's. Research Splitter cables and/or hubs to see if this is possible. Hope this helps!