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NO news is good news - Detection, Measurement and Quantification of Nitric Oxide: Tools for Nitric Oxide Research (Euroscicon Meeting Reports) [Kindle Edition]

Nicholas Warrick , Shara Cohen

Kindle Price: $3.00

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Book Description

Proceedings from the 23rd March 2007 meeting in Hertfordshire, UK

"Nitric oxide (NO), generated by the enzyme nitric oxide synthase (NOS) plays a key role in a diverse range of physiological and pathophysiological conditions within the cardiovascular, immune, reproductive and nervous systems. Since NO is a small, diffusible, highly reactive free radical with a short half-life, and is present in low concentrations, real-time detection of NO is extremely difficult. This meeting will examine methods used to detect and visualise NO-producing cells. Furthermore, direct and indirect techniques used to measure NO will be examined, including among others, the measurement of NOS enzymatic activity, electron paramagnetic resonance (EPR) and NO-sensitive electrodes. Finally, tools that facilitate NO research, such as NOS inhibitors will be examined". Meeting's Chair: Dr Nicholas Warrick - University of Oxford




  • Quantification of Blood and Tissue NO Metabolites by Gas Phase Chemiluminescence: a Guide to NO Man's Land
  • Vascular Nitric Oxide Metabolism
  • Methods for Metabolite Measurement
  • Principle of Chemiluminescence
  • Radiochemical HPLC measurement of nitric oxide synthesis (NOS) activity in vascular tissue
  • EPR-based measurement of reactive nitrogen intermediates in human inflammatory diseases
  • Nitric oxide sensors and their biomedical applications
  • Understanding NO synthase inhibition; potency, selectivity and implications for their pharmacology
  • Endogenously occurring inhibitors of nitric oxide synthase
  • Methylated arginines as NOS inhibitors
  • Measuring ADMA
  • Conditions in which ADMA is elevated
  • Arginine methylation
  • Regulation of ADMA
  • Could ADMA be causative in cardiovascular pathologies?
  • Evaluating endothelial function in humans: From bench to bedside
  • Nitric oxide synthase inhibition and renal injury
  • Quantification of enzyme independent stores of nitric oxide in human skin


  • NO quantification in abnormal and manipulated red blood cells
  • References
  • Application of NO detection using Fe(DETC) spin trap and ESR spectroscopy in pharmacological research
  • Halogenated volatile anestherics (HVA)
  • Flavonoids. Baicalein and luteolin
  • Hypoxia-induced regulation of nitric oxide synthase in cardiac endothelial cells and myocytes
  • Nitrite regulation of energy expenditure in man
  • Nitric oxide inhibits cellular lipid and protein synthesis without energy depletion: a process of chronic diseases due to nitrosothiols
  • NOx concentrations in human brain extracellular fluid and their relationship with energy metabolism: a pilot microdialysis study in acute brain injury

Product Details

  • File Size: 231 KB
  • Print Length: 74 pages
  • Simultaneous Device Usage: Unlimited
  • Publisher: Honnao (June 10, 2012)
  • Sold by: Amazon Digital Services, Inc.
  • Language: English
  • ASIN: B008AL42KA
  • Text-to-Speech: Enabled
  • X-Ray:
  • Word Wise: Not Enabled
  • Lending: Enabled
  • Amazon Best Sellers Rank: #1,859,386 Paid in Kindle Store (See Top 100 Paid in Kindle Store)
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More About the Author

Dr Shara Cohen has been active in the scientific community for over 30 years. She started as a member of the British Association of Young Scientists at the age of 15, obtained her first science degree at 21 and her Immunology PhD at 25. She has been a research scientist and lecturer in prestigious institutions around the world [including Addenbrookes' Hospital (Cambridge), The Kennedy Institute of Rheumatology (London), The Mount Sinai Hospital (New York) and The Royal Postgraduate Medical School (London)]. Shara left mainstream in 2001 research to follow her passion of science communication both within the scientific community and the general public. In 2001 Shara established Euroscicon Ltd (and is still its Managing Director) and in 2005 she founded the Mums in Science Network. She also writes, edits and produces books for both scientists and the layman


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