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Molecular Cloning: A Laboratory Manual (3-Volume Set) (Paperback)

by Joe Sambrook (Author) "THIS PRINT EDITION OF MOLECULAR CLONING is associated with a Web Site (www.MolecularCloning.com) that is evolving into an on-line laboratory manual..." (more)
Key Phrases: desired amplification protocol, autoclave all plasticware, micropipettor equipped, Nucleic Acids Res, Methods Enzymol, Life Technologies (more...)

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Editorial Reviews

Review
"In every kitchen there is at least one indispensable cookbook. Sambrook and Russell's Molecular Cloning: A Laboratory Manual fills the same niche in the laboratory. Like its kitchen counterparts (e.g. Rombeck's Joy of Cooking) Sambrook's Molecular Cloning (MC) has information to help both the inexperienced and the advanced user." --Trends in Neurosciences

Product Description
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today’s biomolecular techniques.

The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.

These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.

The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.

The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Product Details

Paperback: 999 pages
Publisher: Cold Spring Harbor Laboratory Press; 3 Lab edition (January 15, 2001)
Language: English
ISBN-10: 0879695773
ISBN-13: 978-0879695774
Product Dimensions: 13.8 x 9.2 x 4 inches
Shipping Weight: 13.4 pounds (View shipping rates and policies)

Average Customer Review: 4.4 out of 5 stars See all reviews (16 customer reviews)

Amazon.com Sales Rank: #48,356 in Books (See Bestsellers in Books)

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#12 in	Books > Science > Medicine > Basic Science > Genetics
#14 in	Books > Professional & Technical > Medical > Basic Sciences > Genetics
#17 in	Books > Professional & Technical > Engineering > Bioengineering > Biochemistry

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Inside This Book (learn more)

First Sentence:
THIS PRINT EDITION OF MOLECULAR CLONING is associated with a Web Site (www.MolecularCloning.com) that is evolving into an on-line laboratory manual. Read the first page

Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
desired amplification protocol, autoclave all plasticware, micropipettor equipped, chromogenic screening, protocol require the reagents, top agarose containing, micelle near the meniscus, successful amplification reaction, electrolyte gradient gels, water bath preset, lox amplification buffer, protein imprints, nonrecombinant bacteriophages, suit other types, baths preset, please see the introduction, compatible termini, megaprimer method, thermal cycler programmed, deoxynucleotide primers, unused primers, polynucleoticle kinase, quaternary alkylammonium salts, bidirectional deletions, bulldog binder clips

Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Methods Enzymol, Life Technologies, New York, Saran Wrap, Cold Spring Harbor, Methods Mol, Academic Press, Methods Appl, Additional Reagents Step, Gels Agarose, Radioactive Compounds, New England Biolabs, Master Cycler, United Kingdom, Boehringer Mannheim, Cycle Number Denaturation Annealing Polymerization, Special Equipment Barrier, Purification of Radiolabeled Oligonucleotides, University of Texas Southwestern Medical Center, Dideoxy-mediated Sequencing Reactions Using, Ethanol Phenol, Genome Res, Repeat Step, Screening Expression Libraries Constructed

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Citations (learn more)

This book cites 8 books:

What Mad Pursuit: A Personal View of Scientific Discovery by Francis Crick on page 3, and page 136
Parliament of Fowls: Chaucer's Dream Poem on Partnerships by Geoffrey Chaucer on page 3
George Bernard Shaw by Archibald Henderson on page 3
The Lost Worlds of 2001 by Arthur C. Clarke on page 3
A History of Western Philosophy: The Twentieth Century of Quine and Derrida, Volume V by W.T. Jones on page 3

See all 8 books this book cites

100 books cite this book:

Methods of Enzymology, Volume 164: Ribosomes by Jr. Harry F. Noller on 6 pages
Automated DNA Sequencing and Analysis by Mark D. Adams on 5 pages
Peptide Growth Factors, Part C, Volume 198: Volume 198: Peptide Growth Factors Part C (Methods in Enzymology) by John N. Abelson on 5 pages
Small Gtpases & Their Regulators: Part C, Proteins Involved in Transport (Methods in Enzymology) by John N. Abelson on 5 pages
Differentially Expressed Genes In Plants: A Bench Manual by Axel Kornerup Hansen on 5 pages

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Books on Related Topics (learn more)

Condensed Protocols from Molecular Cloning by Joseph Sambrook

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DNA Microarrays by David Bowtell

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Applied Molecular Genetics by Roger L. Miesfeld

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Bioinformatics by David W. Mount

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What Do Customers Ultimately Buy After Viewing This Item?

	83% buy the item featured on this page: Molecular Cloning: A Laboratory Manual (3-Volume Set) 4.4 out of 5 stars (16) $233.10
	5% buy Condensed Protocols from Molecular Cloning: A Laboratory Manual 4.5 out of 5 stars (2) $89.10
	4% buy Short Protocols in Molecular Biology (2 volume set) 4.0 out of 5 stars (5) $136.89
	4% buy At the Bench: A Laboratory Navigator 4.6 out of 5 stars (14) $44.84

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Customer Reviews

16 Reviews

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4.4 out of 5 stars (16 customer reviews)

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Most Helpful Customer Reviews

23 of 23 people found the following review helpful:

5.0 out of 5 stars Excellent reference for all, March 9, 2002

By	Dr. Lee D. Carlson (Baltimore, Maryland USA) - See all my reviews (TOP 100 REVIEWER) (REAL NAME)

In this 3 volume set of books the authors summarize the most important laboratory protocols for DNA analysis and cloning. As someone involved in computational biology and mathematical gene sequence analysis, I was needing such a summary to get an idea of just how genetic engineering is actually practiced in the laboratory. The book is definitely written for those readers that are very experienced in these "wet" techniques, but it still could be perused profitably by anyone who is curious about genetic engineering. There is also an excellent website that owners of the books can go to and search for protocols and obtain updates and additions to the protocols.

At the beginning of each chapter, the authors give an introduction to the protocols and this is of an enormous help to those readers with only rudimentary acquaintance with the laboratory procedures. Typically, this introduction contains an historical summary of the procedures as they were developed or discovered. One can only marvel at the ingenuity of the discoverers of these techniques. These introductions are fairly straightforward to read, even for those that are not experts in biochemistry.

At the end of each chapter, the authors include an "information panel" that gives a more in-depth view of the biochemistry or genetics behind the procedures. These are summaries and are highly specialized, and are again meant for experienced readers. A very lengthy list of references is also included at the end of each chapter.

Becuase of the size of this collection, space here does not permit a detailed review, so I will list some of the areas that I thought were particularly interesting or well-written (these coming from the introduction or the information panels only): 1. The DNA synthesis at the colE1 replicon and the interaction between RNAI and RNAII. 2. The discussion of electroporation and the physics behind this technique to introduce DNA into eukaryotic cells. 3. The discussion on the discovery of bacteriophage lambda. 4. The discussion (with diagram), of the assembly pathway of bacteriophage lambda. 5. The summary of the early analysis of DNA using electrophoresis and the different pulsed-field configurations used. 6. The anecdote on the discovery of the polymerase chain reaction. 7. The short discussion on computer-assisted design of oligonucleotide primers. 8. The discussion of oligonucleotide synthesis. 9. The flowchart detailing the preparing and screening of a cDNA library. 10. The history of the development of the methods to synthesize and clone cDNAs. 11. The detailed discussion of the molecular cloning of double-stranded cDNA. 12. The discussion on the methods to validate clones of cDNA. 13. The discussion on magnetic beads for affinity purification. 14. The discussion on the history of DNA sequencing and the different techniques to accomplish it, particularly the information panel on automated DNA sequencing. 15. The discussion of the different types of mutagenesis and the different methods for accomplishing it. 18. The fascinating discussion of how to introduce cloned genes into mammalian cells. 19. The discussion on the steps involved in DNA footprinting. 20 The discussion on green flourescent protein and its use as a fusion tag. 21. The discussion on the use of surface plasmon resonance spectroscopy.

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9 of 9 people found the following review helpful:

3.0 out of 5 stars a formerly essential classic, November 23, 2005

By	W. D. Dotson "Doc Dave" (Georgia) - See all my reviews (REAL NAME)

For many years the previous edition of this set was an essential reference in molecular biology labs. At present however, there are too many good protocol books out there to really make this argument. The book is pretty strong in explaining theory, and answering the question of why certain procedures are either necessary, useful, or worthless, however it is not as practical as many other books, such as Short Protocols. Still a good reference overall, but no longer stands alone, and I recommend checking out as much of the competition as possible before deciding whether to make the investment in it.

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8 of 8 people found the following review helpful:

5.0 out of 5 stars A must for molecular biologists, December 16, 2001

By	John Unknown "hammett" (Bethesda, MD, USA) - See all my reviews

I love this book, however some areas are better covered in Current Protocols in Molecular Biology, and vice versa. So I would recommend having both.

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Most Recent Customer Reviews

3.0 out of 5 stars Molecular Cloning Manual
The books arrived in good condition, but it took a long time to arrive here.

Published 11 months ago by UWECuser

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