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Edvotek 112 Laboratory Kit 6 - Analysis of Eco RI Cleavage Patterns of Lambda DNA for 8 Gels


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Specifications for this item
Brand Name Edvotek Inc
Number of Items 1
Part Number 112 EDVA7
UNSPSC Code 60101733



WARNING:
CHOKING HAZARD -- Small parts. Not for children under 3 yrs.

Product Features

  • For 8 gels; complete in 60 minutes
  • Kit includes: instructions, Ready-to-Load DNA samples, UltraSpec-Agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain Blue and FlashBlue stain
  • Kit includes: calibrated pipet, 100 ml graduated cylinder, and microtipped transfer pipettes
  • All you need: electrophoresis apparatus, power supply, automatic micropipet and tips,balance, microwave or hot plate, 65 degree C waterbath, visualization system
  • This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences

Product Description

This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments. Kit includes: instructions, Ready-to-Load DNA samples, UltraSpec-Agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain Blue and FlashBlue stain, calibrated pipet, 100 ml graduated cylinder, and microtipped transfer pipettes. All you need: electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, 65 degree C waterbath, visualization system. DNA samples are stable at room temperature. However, if the experiment will not be conducted within one month of receipt, it is recommended that the DNA samples be stored in the refrigerator. DNA samples do not require heating prior to gel loading. Ready-to-Load DNA samples for electrophoresis: A: Standard DNA Fragments, B: Lambda DNA cut with Eco RI, C: Lambda DNA (uncut).

Product Details

EDVO-Kit #112/AP09 Safety Data Sheets (SDS) [PDF]| EDVO-Kit #112/AP09 Protocol [PDF]| Material Safety Data Sheet [193kb PDF]

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