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Millipore UFC5100BK Regenerated Cellulose Amicon Ultra Centrifugal Filter Ultracel-100 Membrane for Protein Purification and Concentration, 0.5mL Capacity, 100kDa NMWL, 10.8mm Diameter x 49.9mm Length, 1 sq cm Membrane (Pack of 500)

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Specifications for this item
Brand Name Millipore
Part Number UFC5100BK
Material Type Cellulose
Number of Items 500
Outside Diameter 10.8  mm
Overall Length 49.9  mm
UNSPSC Code 41104916

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Product Features

  • Concentrates 500L down to 15L; concentration factor 25 x to 30 x; high sample recovery (greater than 90 percent of dilute starting solution); fast processing time - 10 to 30 minutes^Consistent recoveries thanks to the reverse spin capability; reverse spin capability provides consistent recoveries^Purification of macromolecular components found in tissue culture extracts or cell lysates^Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC^Desalting, buffer exchange and protein dialysis; size exclusion and protein fractionation

Product Description

Trade Name: Amicon. Key Applications: Protein Purification, Western Blotting and Protein Extraction. Application Notes: Fixed angle rotor: 14,000 x g for concentration spin; 1,000 x g for reverse spin. Length, cm (in): 4.99 (0.196). Filter Material: Regenerated Cellulose. Centrifugal Application: Protein Concentration and Desalting. Centrifugal Filter Unit Type: Protein Sample Prep. Color Code: Clear. Media Brand Name: Ultracel. Minimum Final Concentrate Volume,�L: 15. Filter Type: Ultrafiltration. Length,mm: 49.9. NMWL, kDa: 100. Replaces: 42424. Diameter,mm: 10.8. Filter Code: YML. Volume, mL: 0.5. Filtration Area, cm squared: 1. Device Material: Housing: Styrene/butadiene and Cap and Tube: Polypropylene. Material Supplied: The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample. Maximum initial sample volume,�L: 500. Typical final concentration volume,�L: 15 - 20. Recommended relative centrifugal force: 14,000 x g for concentration spin; 1,000 x g for reverse spin. Maximum relative centrifugal force: 15,000 x g. Active membrane area, cm squared: 1. Hold up volume,�L: <=1. Filter device: Copolymer styrene/butadiene. Membrane: Millipore Ultracell low-binding regenerated cellulose. Collection tube: Polypropylene. The ultrafiltration membranes in Amicon Ultra-0.5 mL devices contain trace amounts of the following:: Amicon Ultra 3K devices: glycerine; Amicon Ultra 10K device: triethylene glycol (TEG). If these materials interfere with analysis, pre-rinse the device with buffer or Milli-Q water. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q water. Filter device: Diameter: 9.4mm and 29.6mm Length. Tube: 10.8mm Diameter and 43.0mm Length. Filter device and tube: 49.9mm Length (concentration mode; device in tube); 37.3mm Length (reverse spin; device upside down in tube).

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