This laboratory manual differs from many of the numerous others currently on the market in that is explains the "Whys" as well as the "Hows" of the most commonly used molecular techniques. ... It is absolutely jam packed full of those little titbits of information that friendly Post Docs occasionally feel inclined to share with students. ... I liked this book, ... (it) is principally aimed at students ... and the emphasis is very definitely on making techniques accessible to these people. ... this particular book more than adequately fills the niche it is intended to cover.
Kate E. Plant, Univ. of Oxford, UK in Journal of Cell Science 2000, 113
From the Back Cover
Step-by-step protocols and practical notes are provided. The distinguishing feature of this manual compared to others is the detailed explanation of the theoretical mechanisms of each step and the discussion of the importance of the techniques. In addition, each protocol includes an indication of the time and expense involved in its application. This knowledge will enable the users to design their own modifications or to adapt the method to different systems.
Dr. Surzycki has been teaching undergraduate courses and leading workshops in introductory Molecular Biology for many years, during which time he has extensively modified and refined the techniques he has described in this manual.