Yes I have, I have a Mac Book Air and it only has one USB. The problem with this DVD player is you need both of them to work. It is possible to plug in one to put the disk in, unplug it, plug in the other one, and make it work or you could try looking for one with only one USB
I have multiple USB ports but I have used it with just the main one. The purpose of the extra one is speed as you would be transferring data or whatever using two pathways. It isn't necessary though and works just fine without it.
I have one regular 2.0 USB & one 3.0 USB on my ultra book. I plugged the burner in to the 2.0. I just unplug the burner when I need to use the 2.0 for something else. I usually don't need both at the exact same time so it works. Don't usually use the 3.0 for flash drives because they are configured on 2.0 usb drives. The directions show using both but that is misleading. You only need one to use the burner. The other end is for optional power boost according to the directions.
If you mean can you use this as reader and writer, yes. I often use it that way, though I presently have 2 drives connected. If you mean can you plug it in as needed to use it I see no reason why not. Any modern OS should recognize it each time.
This product only needs to be connected using one USB connector. Connect to the computer with the USB connector that is in a straight line with the connector cable. The other UBS connector is not needed.